AB314367

Human MX1 ELISA Kit

SimpleStep
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Human MX1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MX1 with a sensitivity of 14.15 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Interferon-induced GTP-binding protein Mx1, Interferon-induced protein p78, Interferon-regulated resistance GTP-binding protein MxA, Myxoma resistance protein 1, Myxovirus resistance protein 1, IFI-78K, MX1

3 Images

Sandwich ELISA - Human MX1 ELISA Kit (AB314367)

sELISA

Supplier Data

Sandwich ELISA - Human MX1 ELISA Kit (AB314367)

Comparison of native human MX1 concentration in treated versus mock treated Daudi cell extracts

Daudi cells were treated overnight with 20 µg/mL human IFN-alpha then followed by protein extraction. Mock control Daudi cells accompanied cell treatment. Concentrations of human MX1 were measured in duplicate and interpolated against the standard curve. Results show the mean human MX1 was not detected (ND) in the mock samples compared to 2883 pg/mL in IFN-alpha treated samples.

sELISA

Supplier Data

Sandwich ELISA - Human MX1 ELISA Kit (AB314367)

Interpolated concentrations of human MX1 in Daudi + IFN alpha cell extract and human tonsil tissue extract

Interpolated concentration of native MX1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 25 µg/mL Daudi + IFN alpha Extract, and 50 µg/mL tonsil tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human MX1 ELISA Kit (AB314367)

Example of human MX1 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell Lysate, Tissue Extracts

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 14.15 pg/mL

検出範囲

70.313 - 4500 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human MX1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MX1 protein in Cell Lysate, Tissue extracts. Quantitate Human MX1 with 14.15 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" } ]

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回収率

[ { "sample": "Cell Lysate", "range": "84 - 99 %", "average": "= 93" }, { "sample": "Tissue Extracts", "range": "98 - 113 %", "average": "= 105" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Interferon-induced dynamin-like GTPase with antiviral activity against a wide range of RNA viruses and some DNA viruses. Its target viruses include negative-stranded RNA viruses and HBV through binding and inactivation of their ribonucleocapsid. May also antagonize reoviridae and asfarviridae replication. Inhibits thogoto virus (THOV) replication by preventing the nuclear import of viral nucleocapsids. Inhibits La Crosse virus (LACV) replication by sequestering viral nucleoprotein in perinuclear complexes, preventing genome amplification, budding, and egress. Inhibits influenza A virus (IAV) replication by decreasing or delaying NP synthesis and by blocking endocytic traffic of incoming virus particles. Enhances ER stress-mediated cell death after influenza virus infection. May regulate the calcium channel activity of TRPCs.

See full target information MX1

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websiteProtocolBooklet

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Abcam product promise

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Human MX1 ELISA Kit