Human MIP2 ELISA Kit (CXCL2) (ab184862)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 pg/ml
- Range: 2.3 pg/ml - 150 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human MIP2 ELISA Kit (CXCL2)
CXCL2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Human Serum 24 2.81% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Human Serum 24 3.46% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 1 pg/ml -
検出範囲
2.3 pg/ml - 150 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 101.3 89.9% - 110.09% Cell culture media 106.57 97.09% - 116.04% Hep Plasma 100.07 87.61% - 116.98% EDTA Plasma 96.92 94.06% - 100.01% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human MIP2 (CXCL2) ELISA kit (ab184862) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP2 protein in in human serum, plasma, and cell culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate human MIP2 with 1 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
特記事項
Macrophage inflammatory protein 2 (MIP2) otherwise known as CXCL2 GRO-beta| or Hematopoietic synergistic factor is a 7.9 kDa heparin-binding chemokine that has potent effects in the response to inflammation and induction of peripheral tolerance. MIP2 shares 70% sequence similarity with mouse and rat MIP2. It is secreted by activated monocytes neutrophils and inflamed mucosal epithelial cells in response to inflammatory stimuli such as IL-1β. MIP2 recruits granulocytic neutrophils and macrophages at sights of inflammation| and causes degranulation of these effector cells at the inflammatory site. It has also been hypothesized that MIP2 acts to synergize the effects of Granulocyte macrophage colony-stimulating factor (GM-CSF) and Macrophage colony-stimulating factor (M-CSF) leading to a larger recruitment of neutrophils and macrophages at the site of inflammation. Research has also suggested that MIP2 is involved in tolerogenic pathways as a lack of the MIP2 receptors CXCR1 and CXCR2 are associated with peripheral tolerance. Clinically. MIP2 is being used as a bone marrow transplant regimen to mobilize peripheral blood hematopoietic stem cells (PBSCs). Mobilization by MIP2 therapy in combination with G-CSF has been associated with faster immune cell reconstitution after transplantation.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human MIP2 Capture Antibody 1 x 600µl 10X Human MIP2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human MIP2 Lyophilized Recombinant Protein (ab9842) 2 vials Plate Seal 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C-X-C motif chemokine 2
- Chemokine (C X C motif) ligand 2
- Chemokine, CXC motif, ligand 2
see all -
参照データベース
- Entrez Gene: 2920 Human
- Omim: 139110 Human
- SwissProt: P19875 Human
- Unigene: 75765 Human
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184862の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
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Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Other - Human MIP2 ELISA Kit (CXCL2) (ab184862)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Linearity of dilution for biologicals.Interpolated values corrected by dilution factor (mean +/- SD) are graphed.
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Typical Standard CurveExample of MIP2 standard curve in Sample Diluent NS using ab184862
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Typical Standard CurveExample of MIP2 standard curve in Sample Diluent 25BP using ab184862
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Specificity of MIP2 on stimulated and non stimulated media supernatants.Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin and 100 µg/mL streptomycin. Cells were cultured for 2 days at 37˚C in the presence or absence of PHA. The concentrations of MIP2 were interpolated from the calibration curve and corrected for sample dilution. The mean MIP2 concentration was 67 pg/mL on unstimulated PBMC supernatants and 773 pg/mL on stimulated PBMCs supernatants.
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Levels of MIP2 in human serum samples.Human MIP2 levels in individual healthy donors. Ten individual healthy donors were evaluated for the presence of MIP2 in serum using this assay. Results were interpolated from the standard curve in Sample Diluent 25BP and corrected for sample dilution (1:4). The mean level of Human MIP2 was found at 84.757 pg/mL with a range of 26.767 – 266.256 pg/mL.
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Sandwich ELISA - Human MIP2 ELISA Kit (CXCL2) (ab184862)To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab184862 は 12 報の論文で使用されています。
- Han B et al. A chemokine regulatory loop induces cholesterol synthesis in lung-colonizing triple-negative breast cancer cells to fuel metastatic growth. Mol Ther 30:672-687 (2022). PubMed: 34274535
- Chen J et al. Targeted neutrophil-mimetic liposomes promote cardiac repair by adsorbing proinflammatory cytokines and regulating the immune microenvironment. J Nanobiotechnology 20:218 (2022). PubMed: 35525963
- Sukhanov S et al. The SGLT2 inhibitor Empagliflozin attenuates interleukin-17A-induced human aortic smooth muscle cell proliferation and migration by targeting TRAF3IP2/ROS/NLRP3/Caspase-1-dependent IL-1β and IL-18 secretion. Cell Signal 77:109825 (2021). PubMed: 33160017
- Wang J et al. Crocin has pharmacological effects against the pathological behavior of colon cancer cells by interacting with the STAT3 signaling pathway. Exp Ther Med 19:1297-1303 (2020). PubMed: 32010302
- Yang J et al. Characteristics of CXCL2 expression in coronary atherosclerosis and negative regulation by microRNA-421. J Int Med Res 48:300060519896150 (2020). PubMed: 32212883