Human MICA ELISA Kit (ab59569)
Key features and details
- Sensitivity: 123 pg/ml
- Range: 156.25 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human MICA ELISA Kit
MICA キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% A 6 2214.6pg/ml 50.3 2.3% B 6 1102.1pg/ml 39.5 3.6% C 6 641.6pg/ml 47.7 7.4% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% A 18 2416pg/ml 187.1 7.7% B 16 1200pg/ml 105.2 8.8% C 18 627pg/ml 50.9 8.1% -
サンプルの種類
Cell culture supernatant -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 123 pg/ml -
検出範囲
156.25 pg/ml - 5000 pg/ml -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s MICA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of MHC class 1 chain-related gene A glycoprotein, in cell culture supernatants and buffered solutions
A monoclonal antibody specific for MICA has been coated onto the wells of the microtiter strips provided. Samples, including standards of known MICA concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for MICA are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of MICA present in the samples.
This kit will recognize both endogenous and recombinant Human MICA.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 2 x 96 tests 1 x 96 tests 1X Standard Diluent Buffer 2 x 25ml 1 x 25ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml Biotinylated anti-MICA (MHC Class I alpha) Red 2 x 400µl 1 x 400µl Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml HRP Diluent Red 1 x 23ml 1 x 12ml MICA Microplate (12 x 8 well strips) 2 units 1 unit MICA Standard (lyophilized) Yellow 4 vials 2 vials Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
研究分野
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関連性
The MHC class I chain-related (MIC) proteins are related to the Major histocompatibility complex (MHC) class I proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. The MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. MICA is present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system. -
細胞内局在
Plasma membrane -
別名
- FLJ60820
- HLA class I antigen
- MGC111087
see all -
参照データベース
- Entrez Gene: 4276 Human
- Omim: 600169 Human
- SwissProt: Q29983 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59569の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab59569.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab59569 は 3 報の論文で使用されています。
- Ferrari de Andrade L et al. Inhibition of MICA and MICB Shedding Elicits NK-Cell-Mediated Immunity against Tumors Resistant to Cytotoxic T Cells. Cancer Immunol Res 8:769-780 (2020). PubMed: 32209637
- Formenti SC et al. Radiotherapy induces responses of lung cancer to CTLA-4 blockade. Nat Med 24:1845-1851 (2018). PubMed: 30397353
- Fu D et al. Metabolic perturbation sensitizes human breast cancer to NK cell-mediated cytotoxicity by increasing the expression of MHC class I chain-related A/B. Oncoimmunology 4:e991228 (2015). PubMed: 25949910