AB59569

Human MICA ELISA Kit

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(3 Publications)

Human MICA ELISA Kit is a sandwich ELISA designed to quantify Human MICA with a sensitivity of 123 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 156.25 - 5000 pg/mL

別名を表示する

PERB11.1, MICA, MHC class I polypeptide-related sequence A, MIC-A

1 Images

ELISA

Unknown

ELISA - Human MICA ELISA Kit (AB59569)

Representative Standard Curve using ab59569.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture supernatant

交差種

Human

アッセイタイプ

Sandwich (quantitative)

検出感度

= 123 pg/mL

検出範囲

156.25 - 5000 pg/mL

アッセイ時間

3h 45m

アッセイプラットフォーム

Microplate

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Reactivity data

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製品の詳細

Human MICA ELISA Kit ab59569 is a sandwich ELISA to measure Human MICA in cell culture supernatant with a sensitivity of 123 pg/ml.

How the assay works

A monoclonal antibody specific for MICA has been coated onto the wells of the microtiter strips provided. Samples, including standards of known MICA concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for MICA are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of MICA present in the samples.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human MICA ELISA Kit ab59569 protocol summary

1. Add standard or sample to each well used
2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperature
3. Aspirate and wash each well
4. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature
5. Aspirate and wash each well.
6. Add the TMB Solution to each well until color develops and then add the Stop Solution
7. Immediately begin recording the color development

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精度

[ { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 16, "mean": "1200 pg/mL", "standardDeviation": "105.2", "coefficientOfVariability": "8.8" }, { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 18, "mean": "2416 pg/mL", "standardDeviation": "187.1", "coefficientOfVariability": "7.7" }, { "reproducibilityType": "Inter", "sample": "Sample C", "replicates": 18, "mean": "627 pg/mL", "standardDeviation": "50.9", "coefficientOfVariability": "8.1" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 6, "mean": "2214.6 pg/mL", "standardDeviation": "50.3", "coefficientOfVariability": "2.3" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 6, "mean": "1102.1 pg/mL", "standardDeviation": "39.5", "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Intra", "sample": "Sample C", "replicates": 6, "mean": "641.6 pg/mL", "standardDeviation": "47.7", "coefficientOfVariability": "7.4" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MICA also known as MHC class I polypeptide-related sequence A is a protein encoded by the MICA gene. It has a molecular weight of approximately 62 kDa. MICA is a stress-induced ligand expressed on the surface of various cell types including epithelial cells fibroblasts and some tumor cells. This protein is part of the MHC class I chain-related (MIC) family which plays an important role in the immune system by interacting with natural killer (NK) cells and certain T cells.

Biological function summary

The MICA protein acts as a ligand for the NKG2D receptor an activating receptor found on NK cells and some T-cell subsets. MICA does not require expression as part of a complex with β2-microglobulin for function. When cells express MICA they signal distress allowing immune cells to target and destroy potentially harmful cells such as those infected with viruses or transformed into cancer cells. This interaction is an important component of the immune surveillance system.

Pathways

MICA functions within the natural cytotoxicity effector pathway heavily involved in immune responses. It is also relevant in the DNA damage response pathways. Within these pathways MICA interacts with proteins like NKG2D and plays a part in mediating cellular responses to stress and damage. These interactions enhance the capacity of immune cells to recognize and eliminate abnormal cells.

MICA is associated with conditions such as psoriasis and certain types of cancer. Studies have shown increased levels of soluble MICA in the sera of patients with psoriasis indicating a role in inflammation. Additionally in cancer MICA expression affects tumor immunity connecting to the NKG2D receptor to influence immune cell targeting and destruction of tumor cells. Researchers use tools such as MICA ELISA kits and imaging techniques to study its role in these diseases.

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製品プロトコール

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ターゲットの情報

Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells (PubMed : 11491531, PubMed : 11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production (PubMed : 10426993).

See full target information MICA

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology research 8:769-780 PubMed32209637

2020

Inhibition of MICA and MICB Shedding Elicits NK-Cell-Mediated Immunity against Tumors Resistant to Cytotoxic T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lucas Ferrari de Andrade,Sushil Kumar,Adrienne M Luoma,Yoshinaga Ito,Pedro Henrique Alves da Silva,Deng Pan,Jason W Pyrdol,Charles H Yoon,Kai W Wucherpfennig

Nature medicine 24:1845-1851 PubMed30397353

2018

Radiotherapy induces responses of lung cancer to CTLA-4 blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia C Formenti,Nils-Petter Rudqvist,Encouse Golden,Benjamin Cooper,Erik Wennerberg,Claire Lhuillier,Claire Vanpouille-Box,Kent Friedman,Lucas Ferrari de Andrade,Kai W Wucherpfennig,Adriana Heguy,Naoko Imai,Sacha Gnjatic,Ryan O Emerson,Xi Kathy Zhou,Tuo Zhang,Abraham Chachoua,Sandra Demaria

Oncoimmunology 4:e991228 PubMed25949910

2015

Metabolic perturbation sensitizes human breast cancer to NK cell-mediated cytotoxicity by increasing the expression of MHC class I chain-related A/B.

Applications

Unspecified application

Species

Unspecified reactive species

Dexue Fu,Jean-Francois Geschwind,Swathi Karthikeyan,Eliyahu Miller,Rani Kunjithapatham,Zhijun Wang,Shanmugasundaram Ganapathy-Kanniappan

View all publications

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websiteProtocolBooklet

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Human MICA ELISA Kit