Human MEF2C ELISA Kit (ab302759)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 65.614 pg/ml
- Range: 234.375 pg/ml - 15000 pg/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat, Human
製品の概要
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製品名
Human MEF2C ELISA Kit
MEF2C キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Extract 8 11% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Extract 3 10.4% -
サンプルの種類
Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
65.614 pg/ml -
検出範囲
234.375 pg/ml - 15000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell Lysate 102 95% - 116% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Rat, Human
非交差種: Mouse -
製品の概要
Human MEF2C SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MEF2C protein in human cell extract. Quantitate Human MEF2C with 65.614 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human MEF2C Capture Antibody 1 x 600µl 10X Human MEF2C Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI2 1 x 6ml Human MEF2C Lyophilized Recombinant Protein 1 x 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2. -
組織特異性
Expressed in brain and skeletal muscle. -
関連疾患
Defects in MEF2C are the cause of mental retardation-stereotypic movements-epilepsy and/or cerebral malformations (MRSME) [MIM:613443]. It is a disorder characterized by severe mental retardation, absent speech, hypotonia, poor eye contact and stereotypic movements. Dysmorphic features include high broad forehead with variable small chin, short nose with anteverted nares, large open mouth, upslanted palpebral fissures and prominent eyebrows. Some patients have seizures. -
配列類似性
Belongs to the MEF2 family.
Contains 1 MADS-box domain.
Contains 1 Mef2-type DNA-binding domain. -
発生段階
Expression is highest during the early stages of postnatal development, at later stages levels greatly decrease. -
ドメイン
The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity. -
翻訳後修飾
Phosphorylation on Ser-59 enhances DNA binding activity (By similarity). Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity.
Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation.
Sumoylated on Lys-391 by SUMO2 but not by SUMO1 represses transcriptional activity.
Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation. -
細胞内局在
Nucleus. - Information by UniProt
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別名
- C5DELq14.3
- DEL5q14.3
- MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C)
see all -
参照データベース
- Entrez Gene: 4208 Human
- Entrez Gene: 499497 Rat
- Omim: 600662 Human
- SwissProt: Q06413 Human
- Unigene: 649965 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302759の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Interpolated concentrations of human MEF2C in Ramos extract, Daudi extract, and C6 extract.
Interpolated concentration of native MEF2C was measured in duplicate at different sample concentrations. Undiluted samples are as follows: Ramos extract 400 µg/mL, Daudi extract 100 µg/mL, and C6 extract 200 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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Example of human MEF2C standard curve in 1X Cell Extraction Buffer PTR.Example of human MEF2C standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Simple Step Elisa ProtocolSimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Benefits of Recombinant Antibodies
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab302759 は論文での使用が確認できていません。