AB277420

Human Mannose Receptor ELISA kit

Name: Human Mannose Receptor ELISA kit (ab277420) | Abcam
Brand: Abcam Limited
SKU: AB277420
Price: 135000 JPY
Availability: InStock

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(2 Publications)

Human Mannose Receptor ELISA kit is a Sandwich (quantitative) ELISA for the measurement of Human Mannose Receptor in Human in Biofluids, Cell Culture Media samples.

別名を表示する

CD206, CLEC13D, CLEC13DL, MRC1L1, MRC1, Macrophage mannose receptor 1, MMR, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, Human mannose receptor, Macrophage mannose receptor 1-like protein 1, hMR

1 Images

ELISA - Human Mannose Receptor ELISA kit (AB277420)

ELISA

Supplier Data

ELISA - Human Mannose Receptor ELISA kit (AB277420)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma, Cell culture supernatant, Serum

交差種

Human

アッセイタイプ

Sandwich (quantitative)

検出感度

= 0.41 ng/mL

検出範囲

0.41 - 100 ng/mL

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human Mannose Receptor ELISA Kit (ab277420) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Mannose Receptor in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human Mannose Receptor coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Mannose Receptor present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Mannose Receptor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Mannose Receptor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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精度

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]

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回収率

[ { "sample": "Serum", "range": "83 - 131 %", "average": "= 104.6" }, { "sample": "Plasma", "range": "93 - 142 %", "average": "= 110.3" }, { "sample": "Cell culture media", "range": "91 - 127 %", "average": "= 109.3" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277420-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277420-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277420-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Mannose Receptor coated Microplate", "number":"AB277420-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB277420-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB277420-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277420-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277420-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Mannose Receptor standard protein (Lyophilized)", "number":"AB277420-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Mannose Receptor Antibody", "number":"AB277420-CMP09", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Mannose Receptor also known as CD206 or MRC1 is a type of C-type lectin receptor with a molecular weight of 175 kDa. It is expressed on the surface of macrophages and dendritic cells. This receptor binds to complex carbohydrates like mannose and fucose found on pathogen surfaces playing an important role in their recognition. Mannose Receptors act as endocytic receptors aiding in the internalization and degradation of pathogens.

Biological function summary

The Mannose Receptor functions in pathogen recognition and clearance by binding to specific carbohydrates on the pathogen's surface. It is not part of a larger molecular complex but works independently to facilitate phagocytosis. The receptor is essential in immune responses allowing macrophages and dendritic cells to efficiently capture and process antigens for presentation to T cells. This enables the initiation of adaptive immune responses.

Pathways

Researchers know that the Mannose Receptor plays an important role in immune signaling pathways particularly in antigen processing and presentation. It interacts with proteins involved in these pathways including those necessary for the internalization and degradation of bound antigens. Moreover it works alongside scavenger receptors contributing to the regulation of immune and inflammatory responses.

The Mannose Receptor is often linked to pulmonary tuberculosis and certain types of cancer. When macrophages express this receptor it can modulate immune responses against Mycobacterium tuberculosis by aiding in its recognition. In cancer altered expression of CD206 can indicate tumor-associated macrophages which promote tumor growth by suppressing immune responses. These connections highlight the receptor's potential as a diagnostic marker and therapeutic target.

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製品プロトコール

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ターゲットの情報

Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains.. (Microbial infection) Acts as a phagocytic receptor for bacteria, fungi and other pathogens.. (Microbial infection) Acts as a receptor for Dengue virus envelope protein E.. (Microbial infection) Interacts with Hepatitis B virus envelope protein.

See full target information MRC1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:2057 PubMed37045813

2023

Targeting neuronal lysosomal dysfunction caused by β-glucocerebrosidase deficiency with an enzyme-based brain shuttle construct.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Gehrlein,Vinod Udayar,Nadia Anastasi,Martino L Morella,Iris Ruf,Doris Brugger,Sophia von der Mark,Ralf Thoma,Arne Rufer,Dominik Heer,Nina Pfahler,Anton Jochner,Jens Niewoehner,Luise Wolf,Matthias Fueth,Martin Ebeling,Roberto Villaseñor,Yanping Zhu,Matthew C Deen,Xiaoyang Shan,Zahra Ehsaei,Verdon Taylor,Ellen Sidransky,David J Vocadlo,Per-Ola Freskgård,Ravi Jagasia

Experimental and therapeutic medicine 25:169 PubMed37006880

2023

IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Gao,Shiqi Suo,Jingxia Li,Chunxia Wang,Ranran Deng,Yuxia Hu,Chunzheng Zhang

View all publications

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websiteProtocolBooklet

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Human Mannose Receptor ELISA kit