AB309425

Human LI Cadherin ELISA kit

Name: Human LI Cadherin ELISA kit (ab309425) | Abcam
Brand: Abcam Limited
SKU: AB309425
Price: 135000 JPY
Availability: InStock

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Human LI Cadherin ELISA kit is a Sandwich ELISA for the measurement of Human LI Cadherin in Human in Biofluids, Cell Culture Media samples.

別名を表示する

Cadherin-17, Intestinal peptide-associated transporter HPT-1, Liver-intestine cadherin, LI-cadherin, CDH17

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Sandwich ELISA - Human LI Cadherin ELISA kit (AB309425)

sELISA

Supplier Data

Sandwich ELISA - Human LI Cadherin ELISA kit (AB309425)

Example of standard curve using ab309425. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Plasma, Cell culture supernatant

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 0.14 ng/mL

検出範囲

0.14 - 31.25 ng/mL

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human LI Cadherin ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human LI Cadherin in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human LI Cadherin coated on a 96-well plate.

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回収率

[ { "sample": "Serum", "range": "101 - 110 %", "average": "= 105.3" }, { "sample": "Plasma", "range": "103 - 125 %", "average": "= 113.2" }, { "sample": "Cell culture media", "range": "115 - 133 %", "average": "= 123" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309425-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309425-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309425-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Cadherin-17 Protein Standard", "number":"AB309425-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB309425-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Cadherin-17 Antibody-coated ELISA Plate", "number":"AB309425-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309425-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB309425-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Cadherin-17 Detection Antibody", "number":"AB309425-CMP09", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

LI Cadherin also known as Cadherin-17 is a protein that plays a significant role in cell adhesion specifically in epithelial cells. This protein belongs to the cadherin family and has a molecular mass of approximately 88 kDa. LI Cadherin is mainly expressed in the gastrointestinal tract including areas like the stomach and small intestine. It is found on the surfaces of cells where it facilitates cell-cell interaction by binding with other cadherin proteins on adjacent cells.

Biological function summary

This cadherin protein is important for maintaining the integrity of epithelial layers in the gastrointestinal tract. It contributes to the formation of adherens junctions which are necessary for the maintenance of tissue architecture and cellular communication. As part of a larger protein complex in adherens junctions it interacts with cytoskeletal elements to help stabilize cell structures. This mechanical support ensures that cells remain tightly adhered under various physiological conditions.

Pathways

LI Cadherin is involved in pathways that govern cell adhesion and signal transduction. It participates in the Wnt signaling pathway which is vital for cell proliferation and differentiation. Moreover it interacts with E-cadherin and β-catenin within the Wnt pathway aiding in the regulation of gene expression and cellular morphology. These interactions are important for maintaining normal cell behavior and preventing unwanted cell proliferation.

LI Cadherin's expression has been linked to gastrointestinal cancers particularly gastric and colorectal cancers. Aberrations in cadherin-mediated cell adhesion can lead to tumorigenesis as disrupted cell adhesion can cause increased cell motility and invasion. LI Cadherin also engages with proteins such as α-catenin in tumor cells affecting cellular responses to cancerous transformations. By understanding these relationships researchers aim to develop targeted therapies to address malignancies associated with disrupted cadherin function.

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製品プロトコール

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ターゲットの情報

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.

See full target information Cadherin-17

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websiteProtocolBooklet

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Abcam product promise

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Human LI Cadherin ELISA kit