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リコンビナントSimpleStep

Human LAMP1 ELISA Kit (ab302756)

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Interpolated concentrations of Human LAMP1 in cerebrospinal fluid (CSF).
  • Interpolated concentrations of Human LAMP1 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).
  • Example of Human LAMP1 standard curve in Sample Diluent NS.
  • Simple Step Elisa Protocol
  • Benefits of Recombinant Antibodies

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 10.594 pg/ml
  • Range: 19.531 pg/ml - 1250 pg/ml
  • Sample type: Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human LAMP1 ELISA Kit
    LAMP1 キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Serum 8 4.7%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Serum 3 6.07%

  • サンプルの種類

    Serum, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    10.594 pg/ml

  • 検出範囲

    19.531 pg/ml - 1250 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 91 82% - 99%
    Hep Plasma 98 87% - 108%
    EDTA Plasma 99 94% - 107%
    Cit plasma 100 96% - 103%
    Cerebral Spinal Fluid 110 107% - 112%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Human
    非交差種: Monkey

  • 製品の概要

    Human LAMP1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LAMP1 protein in human serum, plasma, and CSF. Quantitate Human LAMP1 with 10.594 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


    -Single-wash protocol reduces assay time to 90 minutes or less
    -High sensitivity, specificity and reproducibility from superior antibodies
    -Fully validated in biological samples
    -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302756の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration.
特記事項
Sandwich ELISA
Use at an assay dependent concentration.

画像

  • Interpolated concentrations of Human LAMP1 in cerebrospinal fluid (CSF).
    Interpolated concentrations of Human LAMP1 in cerebrospinal fluid (CSF).

    Interpolated concentration of native LAMP1 was measured in duplicate at different sample concentrations. Undiluted samples are 25% CSF.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

  • Interpolated concentrations of Human LAMP1 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).
    Interpolated concentrations of Human LAMP1 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).

    Interpolated concentration of native LAMP1 was measured in duplicate at different sample concentrations. Undiluted samples are 2% serum, plasma (citrate), plasma (EDTA), and plasma (heparin).  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

  • Example of Human LAMP1 standard curve in Sample Diluent NS.
    Example of Human LAMP1 standard curve in Sample Diluent NS.

    Example of Human LAMP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Simple Step Elisa Protocol
    Simple Step Elisa Protocol

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Benefits of Recombinant Antibodies
    Benefits of Recombinant Antibodies

参考文献 (0)

ab302756 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab302756 は論文での使用が確認できていません。

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