Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントSimpleStep

Human LAMP1 ELISA Kit (ab302755)

Submit a review Submit a question
Example of human LAMP1 standard curve in 1X Cell Extraction Buffer PTR.
  • Interpolated concentrations of human LAMP1 in MCF-7 and THP-1 cell extracts.
  • Sandwich ELISA - Human LAMP1 ELISA Kit (ab302755)
  • Benefits of Recombinant Antibodies
  • Sandwich ELISA - Human LAMP1 ELISA Kit (ab302755)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 7.957 pg/ml
  • Range: 19.531 pg/ml - 1250 pg/ml
  • Sample type: Cell Lysate
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human LAMP1 ELISA Kit
    LAMP1 キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Extract 8 6.4%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Extract 3 6.73%

  • サンプルの種類

    Cell Lysate

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    7.957 pg/ml

  • 検出範囲

    19.531 pg/ml - 1250 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell Lysate 113 111% - 114%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Human
    非交差種: Monkey

  • 製品の概要

    Human LAMP1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LAMP1 protein in human cell extracts. Quantitate Human LAMP1 with 7.957 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


    -Single-wash protocol reduces assay time to 90 minutes or less
    -High sensitivity, specificity and reproducibility from superior antibodies
    -Fully validated in biological samples
    -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • 特記事項

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302755の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration.
特記事項
Sandwich ELISA
Use at an assay dependent concentration.

画像

  • Example of human LAMP1 standard curve in 1X Cell Extraction Buffer PTR.
    Example of human LAMP1 standard curve in 1X Cell Extraction Buffer PTR.

    Example of human LAMP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of human LAMP1 in MCF-7 and THP-1 cell extracts.
    Interpolated concentrations of human LAMP1 in MCF-7 and THP-1 cell extracts.

    Interpolated concentration of native LAMP1 was measured in duplicate at different sample concentrations. Undiluted samples are 10 µg/mL MCF-7 cell extract and THP-1 cell extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

  • Sandwich ELISA - Human LAMP1 ELISA Kit (ab302755)
    Sandwich ELISA - Human LAMP1 ELISA Kit (ab302755)
    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
  • Benefits of Recombinant Antibodies
    Benefits of Recombinant Antibodies
  • Sandwich ELISA - Human LAMP1 ELISA Kit (ab302755)
    Sandwich ELISA - Human LAMP1 ELISA Kit (ab302755)
    To learn more about the advantages of SimpleStep ELISA® kits see here.

参考文献 (0)

ab302755 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab302755 は論文での使用が確認できていません。

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

There are currently no Customer reviews or Questions for ab302755.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com