AB246527

Human KLF4 ELISA Kit

Recombinant
SimpleStep
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Human KLF4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human KLF4 with a sensitivity of 35 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

EZF, GKLF, KLF4, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor

3 Images

ELISA

Supplier Data

ELISA - Human KLF4 ELISA Kit (AB246527)

sELISA

Supplier Data

Sandwich ELISA - Human KLF4 ELISA Kit (AB246527)

Interpolated concentrations of native KLF4 in human cell extract based on a 500 μg/mL extract load.

The concentrations of KLF4 were measured in duplicate and interpolated from the KLF4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean KLF4 concentration was determined to be 417.5 pg/mL in NCCIT extract.

sELISA

Supplier Data

Sandwich ELISA - Human KLF4 ELISA Kit (AB246527)

Example of human KLF4 standard curve in 1X Cell Extraction Buffer PTR.

The KLF4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture extracts

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 35 pg/mL

検出範囲

62.5 - 4000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human KLF4 ELISA Kit ab246527 is a rapid single-wash 90-min Sandwich ELISA to measure Human KLF4 in cell culture extracts. This SimpleStep sensitivity is 35 pg/mL.

How the assay works

Human KLF4 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human KLF4 ELISA Kit ab246527 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-KLF4 antibody [EPR20801-119] - BSA and Azide free (Capture) ab252626
- Anti-KLF4 antibody [EPR19590] - BSA and Azide free (Detector) ab252626

KLF4, also known as Krueppel-like factor 4, is an embryonic transcription factor involved in the maintenance of maintaining pluripotency by preventing differentiation. It is also found in post-mitotic cells, where its overexpression inhibits proliferation. KLF4 is currently under investigation as a tumor suppressor given it potent ability to halt proliferation. Altered expression of its gene has been noted in many types of human cancers.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]

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回収率

[ { "sample": "Cell culture extracts", "range": "84 - 125 %", "average": "= 118" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

KLF4 also known as Kruppel-like factor 4 acts as a transcription factor involved in the regulation of gene expression. It has a molecular weight of approximately 55-60 kDa. Researchers often find KLF4 expressed in epithelial tissues such as the skin and gastrointestinal tract. It regulates cellular processes like differentiation proliferation and apoptosis playing a role in maintaining homeostasis and cell identity.

Biological function summary

KLF4 impacts cell differentiation and proliferation through its role as a transcription factor. It functions within several cellular complexes and interacts with other transcription factors to modulate gene expression. KLF4 regulates stem cell maintenance by influencing gene networks involved in self-renewal and differentiation contributing particularly to pluripotency in embryonic stem cells.

Pathways

The transcriptional activity of KLF4 integrates into key signaling pathways like the Wnt/?-catenin and Notch pathways. Within the Wnt/?-catenin pathway KLF4 may cooperate with ?-catenin and TCF/LEF transcription factors to modulate gene targets. Similarly in the Notch signaling pathway KLF4 interacts with pathway components to influence cell fate decisions. These interactions highlight KLF4's central role in orchestrating complex signaling networks.

Mutations or dysregulated expression of KLF4 associates with various conditions including colorectal cancer and skin disorders like atopic dermatitis. In colorectal cancer KLF4 often interacts with other oncogenes and tumor suppressor proteins such as p53 to influence tumorigenesis. In skin disorders altered KLF4 expression can impact skin barrier function and inflammatory responses connecting it with proteins involved in immune response regulation.

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製品プロトコール

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ターゲットの情報

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.

See full target information KLF4

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websiteProtocolBooklet

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Human KLF4 ELISA Kit