AB313977

Human JAM-C ELISA Kit

Name: Human JAM-C ELISA Kit (ab313977) | Abcam
Brand: Abcam Limited
SKU: AB313977
Price: 114000 JPY
Availability: InStock

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Human JAM-C ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human JAM-C in Human in Biofluids, Cell Culture Media samples.

別名を表示する

UNQ859/PRO1868, JAM3, Junctional adhesion molecule C, JAM-C, JAM-2, Junctional adhesion molecule 3, JAM-3, JAMC

1 Images

Sandwich ELISA - Human JAM-C ELISA Kit (AB313977)

sELISA

Supplier Data

Sandwich ELISA - Human JAM-C ELISA Kit (AB313977)

Example of standard curve using ab313977. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Plasma, Cell culture supernatant

交差種

Human

アッセイタイプ

Sandwich (quantitative)

検出感度

= 0.12 ng/mL

検出範囲

0.12 - 30 ng/mL

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human JAM-C ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human JAM-C in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human JAM-C coated on a 96-well plate.

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回収率

[ { "sample": "Serum", "range": "101 - 115 %", "average": "= 108.5" }, { "sample": "Plasma", "range": "91 - 109 %", "average": "= 100.1" }, { "sample": "Cell culture media", "range": "98 - 104 %", "average": "= 103.1" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Junctional adhesion molecule-C (JAM-C) is a cell adhesion molecule belonging to the immunoglobulin superfamily also known as JAM3. It has a mass around 37 kDa. JAM-C is present on endothelial cells various immune cells and some epithelial cells. This protein functions mechanically in maintaining tight junctions that control paracellular permeability and are important in cell-to-cell interactions.

Biological function summary

Junctional adhesion molecule-C plays significant roles in leukocyte migration angiogenesis and the maintenance of vascular endothelial barrier function. JAM-C is part of a larger junctional adhesion molecule complex which includes JAM-A and JAM-B that collectively influence these processes. Its expression in tissues such as the heart and brain suggests specialized roles in physiological functions including cardiovascular regulation and CNS barriers.

Pathways

Junctional adhesion molecule-C is an integral component of the leukocyte transmigration and angiogenesis pathways. These pathways collaborate to allow immune cells to pass through vessel walls a fundamental process in immune surveillance and response. Additionally JAM-C interacts with integrins and other adhesion molecules facilitating cellular communication necessary for tissue repair and growth.

Dysregulation of junctional adhesion molecule-C expression associates with inflammatory diseases and cancer. Altered JAM-C levels have implications in autoimmune disorders where abnormal leukocyte extravasation occurs. Its connection to cancer involves interactions with proteins like VE-cadherin contributing to increased tumor angiogenesis and metastasis making it a potential target for therapeutic intervention in these conditions.

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製品プロトコール

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ターゲットの情報

Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM2 to regulate different cellular processes (PubMed : 11590146, PubMed : 11823489). Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow. At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3 (PubMed : 11590146, PubMed : 24357068). Plays a central role in leukocytes extravasation by facilitating transmigration through the endothelium (By similarity). Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation (By similarity). Also functions as a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) (PubMed : 12208882, PubMed : 15194813). Plays a role in angiogenesis (PubMed : 23255084). Plays a role in the regulation of cell migration (Probable). During myogenesis, it is involved in myocyte fusion (By similarity).. Soluble form of JAM-C. Promotes chemotaxis of vascular endothelial cells and stimulates angiogenesis.

See full target information JAM3

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Human JAM-C ELISA Kit