Human IP-10 ELISA Kit (CXCL10) (ab83700)
Key features and details
- Sensitivity: 5.7 pg/ml
- Range: 6.25 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IP-10 ELISA Kit (CXCL10)
IP10 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% A 6 60.1pg/ml 0.9 1.6% B 6 32.4pg/ml 0.5 1.5% C 6 19.6pg/ml 0.3 1.3% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% A 18 56.9pg/ml 2.7 4.7% B 18 29.9pg/ml 2 6.5% C 18 17.7pg/ml 1.5 8.5% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 5.7 pg/ml -
検出範囲
6.25 pg/ml - 200 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 120 118.7% - 122.4% -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IP-10 (CXCL10) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IP-10 in supernatants, buffered solutions, serum and plasma samples.
A monoclonal antibody specific for IP-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IP-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IP-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IP-10 present in the samples.
This kit will recognize both endogenous and recombinant Human IP-10.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated anti IP-10 2 x 400µl 1 x 400µl Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml HRP Diluent Red 1 x 23ml 1 x 12ml IP-10 Microplate (12 x 8 well strips) 2 units 1 unit IP-10 Standard (Lyophilized) 4 vials 2 vials Plastic Plate Covers 4 units 2 units Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
研究分野
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機能
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Interferon gamma induced factor MOB1, mouse, homolog of
- Interferon gamma induced protein 10
- 10 kDa interferon gamma induced protein
see all -
参照データベース
- Entrez Gene: 3627 Human
- Omim: 147310 Human
- SwissProt: P02778 Human
- Unigene: 632586 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab83700の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab83700
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab83700 は 10 報の論文で使用されています。
- Zhang JW et al. Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway. Gut Microbes 15:2197836 (2023). PubMed: 37017266
- Li D et al. Study on the mechanism of oral administration of tetrandrine during neoadjuvant chemotherapy for colon cancer. Oncol Lett 25:225 (2023). PubMed: 37153045
- Zhu F et al. H1N1 Influenza Virus-Infected Nasal Mucosal Epithelial Progenitor Cells Promote Dendritic Cell Recruitment and Maturation. Front Immunol 13:879575 (2022). PubMed: 35572503
- El-Domyati M et al. Systemic CXCL10 is a predictive biomarker of vitiligo lesional skin infiltration, PUVA, NB-UVB and corticosteroid treatment response and outcome. Arch Dermatol Res N/A:N/A (2021). PubMed: 33866437
- Yang X et al. Inhibition of ITGB1 enhance the anti-tumor effect of cetuximab in colorectal cancer cell. Medicine (Baltimore) 99:e20944 (2020). PubMed: 32629699
- Hosseinkhani B et al. (Sub)populations of extracellular vesicles released by TNF-a -triggered human endothelial cells promote vascular inflammation and monocyte migration. J Extracell Vesicles 9:1801153 (2020). PubMed: 32944190
- Carrasco-Pozo C et al. Hemin Prevents Increased Glycolysis in Macrophages upon Activation: Protection by Microbiota-Derived Metabolites of Polyphenols. Antioxidants (Basel) 9:N/A (2020). PubMed: 33187129
- Ren X et al. Effects of fluconazole on the clinical outcome and immune response in fungal co-infected tuberculosis patients. Microb Pathog 117:148-152 (2018). ELISA . PubMed: 29432913
- Hosseinkhani B et al. Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells. Front Immunol 9:1789 (2018). PubMed: 30131806
- You N et al. COMMD7 activates CXCL10 production by regulating NF-?B and the production of reactive oxygen species. Mol Med Rep 17:6784-6788 (2018). PubMed: 29532873