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Human Interferon gamma ELISA Set (without plates) (ab48490)

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Interferon gamma Human ELISA Set (ab48490)

    製品の概要

    • 製品名

      Human Interferon gamma ELISA Set (without plates)
      Interferon gamma キット 製品一覧

    • サンプルの種類

      Cell culture supernatant, Serum, Plasma

    • アッセイタイプ

      Sandwich (quantitative)

    • 検出感度

      < 5 pg/ml

    • 検出範囲

      12.5 pg/ml - 400 pg/ml

    • 全工程の試験時間

      2h 45m

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Human

    • 製品の概要

      Abcam’s IFNγ Human in vitro ELISA Set is designed for the quantitative measurement of IFNγ in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.


      The Interferon gamma ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.


      This ELISA Set is intended to measure human Interferon gamma in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


      Sensitivity: < 5 pg / ml

    • アプリケーション

      適用あり: Sandwich ELISAmore details

    • 試験プラットフォーム

      Reagents

    製品の特性

    • 保存方法

      Store at +4°C. Please refer to protocols.
    • 内容 ラベル 5 x 96 tests 10 x 96 tests 15 x 96 tests
      Detection biotinylated anti-IFN gamma antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
      IFN gamma Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml
      IFN gamma Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials
      Ready-to-use TMB 2 vials 4 vials 6 vials
      Streptavidin-HRP 1 vial 2 vials 3 vials

    • 研究分野

    • 機能

      Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

    • 組織特異性

      Released primarily from activated T lymphocytes.

    • 関連疾患

      In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

    • 配列類似性

      Belongs to the type II (or gamma) interferon family.

    • 翻訳後修飾

      Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 別名

      • IF 1
      • IFG
      • IFI
      • ifn g
      • IFN gamma
      • IFN immune
      • IFN, immune
      • IFN-gamma
      • IFNG
      • IFNG_HUMAN
      • Immune interferon
      • Interferon gamma
      • Type II Interferon
      see all

    • 参照データベース

      アプリケーション

      The Abpromise guarantee

      Abpromise保証は、 次のテスト済みアプリケーションにおけるab48490の使用に適用されます

      アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

      アプリケーション Abreviews 特記事項
      Sandwich ELISA
      Use at an assay dependent concentration.
      特記事項
      Sandwich ELISA
      Use at an assay dependent concentration.

      画像

      • Interferon gamma Human ELISA Set (ab48490)
        Interferon gamma Human ELISA Set (ab48490)
        IFNgamma Standard curve ranging from 12.5 to 400 pg/mL

      参考文献 (0)

      ab48490 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab48490 は論文での使用が確認できていません。

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