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リコンビナントSimpleStep

Human Interferon gamma ELISA Kit (ab300323)

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Sandwich ELISA - Human Interferon gamma ELISA Kit (ab300323)
  • Sandwich ELISA - Human Interferon gamma ELISA Kit (ab300323)
  • Example of human Interferon-gamma standard curve in Sample Diluent NS.
  • Interpolated concentrations of human Interferon-gamma in stimulated PBMC cell culture supernatant.
  • Interpolated concentrations of Human Interferon-gamma in PBMC cell culture supernatants.
  • Human Interferon-gamma competitor standard curve comparison.
  • Human Interferon gamma ELISA Kit (ab300323)
  • Human Interferon gamma ELISA Kit (ab300323)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.428 pg/ml
  • Range: 7.813 pg/ml - 500 pg/ml
  • Sample type: Cell culture media, Cell culture supernatant, EDTA Plasma, Hep Plasma, Saliva, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human Interferon gamma ELISA Kit
    Interferon gamma キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Supernatant 8 7.4%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Supernatant 3 11.38%

  • サンプルの種類

    Cell culture supernatant, Saliva, Serum, Cell culture media, Hep Plasma, EDTA Plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    3.428 pg/ml

  • 検出範囲

    7.813 pg/ml - 500 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 104 97% - 115%
    Serum 101 96% - 104%
    Hep Plasma 103 93% - 114%
    EDTA Plasma 100 93% - 106%
    Cit plasma 101 95% - 109%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Human Interferon gamma SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Interferon gamma protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Interferon gamma with 3.428 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


    -Single-wash protocol reduces assay time to 90 minutes or less
    -High sensitivity, specificity and reproducibility from superior antibodies
    -Fully validated in biological samples
    -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 96 tests 1 x 384 tests
    10X Human Interferon gamma Capture Antibody 1 x 600µl 1 x 600µl
    10X Human Interferon gamma Detector Antibody 1 x 600µl 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml
    384 well CaptSure™ microplates 0 x 0 unit 1 unit
    Antibody Diluent 4BI 1 x 6ml 1 x 6ml
    Human Interferon gamma Lyophilized Recombinant Protein 2 vials 2 vials
    Plate Seal 1 unit 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 0 x 0 unit
    Stop Solution 1 x 12ml 2 x 12ml
    TMB Development Solution 1 x 12ml 2 x 12ml

  • 研究分野

  • 機能

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

  • 組織特異性

    Released primarily from activated T lymphocytes.

  • 関連疾患

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

  • 配列類似性

    Belongs to the type II (or gamma) interferon family.

  • 翻訳後修飾

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 別名

    • IF 1
    • IFG
    • IFI
    • ifn g
    • IFN gamma
    • IFN immune
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    • Type II Interferon
    see all

  • 参照データベース

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab300323の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.
    特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.

    画像

    • Sandwich ELISA - Human Interferon gamma ELISA Kit (ab300323)
      Sandwich ELISA - Human Interferon gamma ELISA Kit (ab300323)
      Interpolated concentration of native Interferon-gamma was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2.5% stimulated PBMC supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
    • Sandwich ELISA - Human Interferon gamma ELISA Kit (ab300323)
      Sandwich ELISA - Human Interferon gamma ELISA Kit (ab300323)
      Example of human Interferon-gamma standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
    • Example of human Interferon-gamma standard curve in Sample Diluent NS.
      Example of human Interferon-gamma standard curve in Sample Diluent NS.

      Example of human Interferon-gamma standard curve. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of human Interferon-gamma in stimulated PBMC cell culture supernatant.
      Interpolated concentrations of human Interferon-gamma in stimulated PBMC cell culture supernatant.

      Interpolated concentration of native Interferon-gamma was measured in duplicate at different sample concentrations. Undiluted samples are 2.5% stimulated PBMC cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

    • Interpolated concentrations of Human Interferon-gamma in PBMC cell culture supernatants.
      Interpolated concentrations of Human Interferon-gamma in PBMC cell culture supernatants.

      Interpolated concentration of native Interferon-gamma was measured in duplicate in human PBMC cell culture supernatants. Sample dilutions are made in Sample Diluent NS. Human PBMCs were cultured for 46 hours in the presence or absence of 1.5% PHA-M. The concentrations of human Interferon gamma were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the Interferon gamma standard curve. The interpolated values are plotted (mean +/- SD, n=2).

    • Human Interferon-gamma competitor standard curve comparison.
      Human Interferon-gamma competitor standard curve comparison.

      Standard curve comparison between Human Interferon-gamma SimpleStep ELISA kit (ab300323) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

    • Human Interferon gamma ELISA Kit (ab300323)
      Human Interferon gamma ELISA Kit (ab300323)
    • Human Interferon gamma ELISA Kit (ab300323)
      Human Interferon gamma ELISA Kit (ab300323)

    参考文献 (0)

    ab300323 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300323 は論文での使用が確認できていません。

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