AB300323

Human Interferon gamma ELISA Kit

Name: Human Interferon gamma ELISA Kit (ab300323) | Abcam
Brand: Abcam Limited
SKU: AB300323
Price: 98000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Recombinant
SimpleStep
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Human Interferon gamma ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Interferon gamma with a sensitivity of 3.428 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Interferon gamma, IFN-gamma, Immune interferon, IFNG

7 Images

ELISA - Human Interferon gamma ELISA Kit (AB300323)

ELISA

Supplier Data

ELISA - Human Interferon gamma ELISA Kit (AB300323)

sELISA

Lab

Sandwich ELISA - Human Interferon gamma ELISA Kit (AB300323)

Interpolated concentration of native Interferon-gamma was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2.5% stimulated PBMC supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human Interferon gamma ELISA Kit (AB300323)

Example of human Interferon-gamma standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Interferon gamma ELISA Kit (AB300323)

Interpolated concentrations of human Interferon-gamma in stimulated PBMC cell culture supernatant.

Interpolated concentration of native Interferon-gamma was measured in duplicate at different sample concentrations. Undiluted samples are 2.5% stimulated PBMC cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Interferon gamma ELISA Kit (AB300323)

Interpolated concentrations of Human Interferon-gamma in PBMC cell culture supernatants.

Interpolated concentration of native Interferon-gamma was measured in duplicate in human PBMC cell culture supernatants. Sample dilutions are made in Sample Diluent NS. Human PBMCs were cultured for 46 hours in the presence or absence of 1.5% PHA-M. The concentrations of human Interferon gamma were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the Interferon gamma standard curve. The interpolated values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Interferon gamma ELISA Kit (AB300323)

Example of human Interferon-gamma standard curve in Sample Diluent NS.

Example of human Interferon-gamma standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Interferon gamma ELISA Kit (AB300323)

Standard curve comparison between Human Interferon-gamma SimpleStep ELISA kit (ab300323) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture media, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Saliva, Serum

交差種

Human

アッセイタイプ

Sandwich (quantitative)

検出感度

= 3.428 pg/mL

検出範囲

7.813 - 500 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human Interferon gamma ELISA Kit ab300323 is a rapid single-wash 90-min Sandwich ELISA to measure Human Interferon gamma in cell culture media, cell culture supernatant, EDTA plasma, heparin plasma, saliva, serum. This SimpleStep sensitivity is 3.428 pg/mL.

How the assay works

Human Interferon gamma SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Interferon gamma ELISA Kit ab300323 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human Interferon gamma ELISA Kit only requires a maximum of 12.5µL

sampleDesign your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Interferon gamma antibody [EPR24641-55] - BSA and Azide free (Capture) ab289983
- Anti-Interferon gamma antibody [EPR24641-20] - BSA and Azide free (Detector) ab289983

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精度

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.38" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "97 - 115 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "95 - 109 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "93 - 106 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "93 - 114 %", "average": "= 103" }, { "sample": "Serum", "range": "96 - 104 %", "average": "= 101" } ]

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製品内容

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab300323-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB300323-CMP18" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB300323-CMP15" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB300323-CMP16" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB300323-CMP17" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB300323-CMP14" }, { "size":"2 x 1 Vial", "name":"Human Interferon gamma Lyophilized Recombinant Protein", "number":"AB300323-CMP13" }, { "size":"1 x 600 µL", "name":"10X Human Interferon gamma Capture Antibody", "number":"AB300323-CMP11" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB300323-CMP20" }, { "size":"1 x 600 µL", "name":"10X Human Interferon gamma Detector Antibody", "number":"AB300323-CMP12" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB300323-CMP21" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab300323-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB300323-CMP18", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB300323-CMP15", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB300323-CMP17", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB300323-CMP16", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB300323-CMP14", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Interferon gamma Lyophilized Recombinant Protein", "number":"AB300323-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Interferon gamma Capture Antibody", "number":"AB300323-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB300323-CMP20", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB300323-CMP19", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Interferon gamma Detector Antibody", "number":"AB300323-CMP12", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-?) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-? has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-? binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.

Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-? is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naпve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-? is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-? also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-? is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-? are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-? and its interactions can be key in developing therapeutic approaches against these conditions.

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製品プロトコール

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ターゲットの情報

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 16914093, PubMed : 8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed : 8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed : 16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed : 8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed : 8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed : 11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed : 7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).

See full target information IFNG

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文献 (1)

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Human Interferon gamma ELISA Kit