AB213479

Human Interferon alpha 1 ELISA Kit

Recombinant
SimpleStep
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(9 Publications)

Human Interferon alpha 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Interferon alpha 1 with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

別名を表示する

IFNA13, IFNA1, Interferon alpha-1/13, IFN-alpha-1/13, Interferon alpha-D, LeIF D

6 Images

ELISA - Human Interferon alpha 1 ELISA Kit (AB213479)

ELISA

Supplier Data

ELISA - Human Interferon alpha 1 ELISA Kit (AB213479)

sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit (AB213479)

Interpolated concentrations of Interferon alpha-1 in extract of HEK293T cells overexpressing human Interferon alpha-1 based on a 200 ng/mL extract load.

The concentrations of Interferon alpha-1 were measured in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-1 concentration was determined to be 126 pg/mL in extract of HEK293T cells overexpressing Interferon alpha-1.

sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit (AB213479)

Interpolated concentrations of native Interferon alpha-1 in WHO International Standard INTERFERON ALPHA (Human leukocyte-derived) NIBSC 94/784 based on a 75 IU/mL load.

The concentrations of Interferon alpha-1 were measured in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-1 concentration was determined to be 427.9 pg/mL in 75 IU/mL WHO International Standard INTERFERON ALPHA (Human leukocyte-derived) NIBSC 94/784.

sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit (AB213479)

Interpolated concentrations of Interferon alpha-1 in extracts of HEK293T cells overexpressing human Interferon alpha-1 and HEK293T cells transfected with the associated empty vector.

The concentrations of Interferon alpha-1 were measured in three different dilutions in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng Interferon alpha-1 per mg of extract (mean +/- SD, n=3). Interferon alpha-1 concentration was determined to be 647.6 ng/mg in extract of HEK293T cells overexpressing Interferon alpha-1. Values for the empty vector were below the minimum detectable dose.

sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit (AB213479)

Example of human Interferon alpha-1 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit (AB213479)

Example of human Interferon alpha-1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 1 pg/mL

検出範囲

4.68 - 300 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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製品の詳細

Human Interferon alpha 1 ELISA Kit ab213479 is a rapid single-wash 90-min Sandwich ELISA to measure Human Interferon alpha 1 in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 1 pg/mL.

How the assay works

Human Interferon alpha 1 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Interferon alpha 1 ELISA Kit ab213479 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Interferon alpha 1 antibody [EPR18694-27] - BSA and Azide free (Capture) ab242952
- Anti-Interferon alpha 1 antibody [EPR18694-3] - BSA and Azide free (Detector) ab242984

Interferon alpha-1 belongs to a group of functionally related but distinct proteins of interferon alpha family that share around 80% identity of their amino acid sequences. Members of the interferon-alpha family have both anti-viral and immunomodulatory activities on target cells.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]

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回収率

[ { "sample": "Serum", "range": "87 - 92 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "81 - 90 %", "average": "= 87" }, { "sample": "Cell culture extracts", "range": "102 - 108 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "85 - 98 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "76 - 78 %", "average": "= 77" }, { "sample": "Cell culture media", "range": "92 - 94 %", "average": "= 93" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Interferon alpha 1 (IFN-α1) is a type I interferon with significant antiviral and immunomodulatory activities. Alternate names include interferon alpha and IFNA1. This protein weighs approximately 19 kDa and expresses predominantly in leukocytes. It interacts with specific cell surface receptors to initiate various protective mechanisms in the cell. Researchers often study IFN-alpha-1 protein using techniques like interferon alpha ELISA and IFN-α ELISA for its effective quantification in experimental analyses.

Biological function summary

Interferon alpha 1 functions as an important player in the immune response against viral infections. It belongs to a family of cytokines that signal through the JAK-STAT pathway to induce the expression of interferon-stimulated genes (ISGs). IFN-α1 operates independently and does not form large complexes with other proteins ensuring a focused response to pathogens. This protein's role is critical for the initiation of immune responses and limiting the spread of viruses within the host organism.

Pathways

Interferon alpha 1 activates intracellular signaling cascades mainly the JAK-STAT pathway and NF-κB pathway. These pathways have important roles in cellular defense mechanisms. In the JAK-STAT pathway IFN-α1 interacts closely with other interferons and cytokines to enhance antiviral states. Through these pathways it shares functional relationships with proteins such as STAT1 and IRF7 which are critical for mounting effective antiviral defenses.

Interferon alpha 1 relates closely to conditions like hepatitis C and multiple sclerosis. It has therapeutic applications in these diseases due to its immunoregulatory effects. IFN-α1 interacts with various immune system components including major histocompatibility complex (MHC) proteins to modulate immune responses. Abnormalities in IFN-α1 signaling or expression can exacerbate inflammatory and autoimmune disorders linking it to both protective and pathological roles in human health.

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製品プロトコール

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ターゲットの情報

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.

See full target information IFNA1

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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40508201

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SARS-CoV-2-Derived RNA Fragment Induces Myocardial Dysfunction via siRNA-like Suppression of Mitochondrial ATP Synthase.

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CD169 classical monocyte as an important participant in Graves' ophthalmopathy through CXCL12-CXCR4 axis.

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Dongliang Wang,Jie Ling,RongQiang Tan,Huishi Wang,Yixin Qu,Xingyi Li,Jinshan Lin,Qikai Zhang,Qiuling Hu,Zhong Liu,Zhaojing Lu,Yuheng Lin,Li Sun,Dingqiao Wang,Ming Zhou,Zhuoxing Shi,Wuyou Gao,Huijing Ye,Xianchai Lin

The Tohoku journal of experimental medicine 260:263-271 PubMed37081619

2023

Predictive Values of Blood Type I and Type II Interferon Production for Disease Activity and Clinical Response to TNF-α Blocking Therapy in Patients with Ankylosing Spondylitis.

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Yulan Hu,Bo Lou,Zhonghua Jiang,Chunchu Yu

Scientific reports 12:17778 PubMed36273032

2022

Vitamin D enhances type I IFN signaling in COVID-19 patients.

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Shirin Hafezi,Fatemeh Saheb Sharif-Askari,Narjes Saheb Sharif-Askari,Hawra Ali Hussain Alsayed,Habiba Alsafar,Fatme Al Anouti,Qutayba Hamid,Rabih Halwani

Frontiers in medicine 9:896409 PubMed36160124

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Alterations in bile acids as metabolic signatures in the patients with human adenovirus type 7 infection.

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Wen Xu,Juan Du,Ting-Ting Wei,Lin-Yi Chen,Xin-Xin Yang,Tu Bo,Han-Yu Liu,Ming-Zhu Xie,Tian-Shuo Zhao,Jun-Lian Yang,Fuqiang Cui,Wei-Wei Chen,Qing-Bin Lu

iScience 25:104193 PubMed35479410

2022

EMT alterations in the solute carrier landscape uncover SLC22A10/A15 imposed vulnerabilities in pancreatic cancer.

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Debasis Nayak,Brenna Weadick,Avinash K Persaud,Radhika Raj,Reena Shakya,Junan Li,Moray J Campbell,Rajgopal Govindarajan

Nature communications 10:1780 PubMed30992428

2019

The role of platelets in mediating a response to human influenza infection.

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Milka Koupenova,Heather A Corkrey,Olga Vitseva,Giorgia Manni,Catherine J Pang,Lauren Clancy,Chen Yao,Jeffrey Rade,Daniel Levy,Jennifer P Wang,Robert W Finberg,Evelyn A Kurt-Jones,Jane E Freedman

PLoS pathogens 14:e1007174 PubMed30071107

2018

Alcohol enhances type 1 interferon-α production and mortality in young mice infected with Mycobacterium tuberculosis.

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Deepak Tripathi,Elwyn Welch,Satyanarayana Swamy Cheekatla,Rajesh Kumar Radhakrishnan,Sambasivan Venkatasubramanian,Padmaja Paidipally,Abhinav Van,Buka Samten,Kamakshi P Devalraju,Venkata Sanjeev Kumar Neela,Vijaya Lakshmi Valluri,Carol Mason,Steve Nelson,Ramakrishna Vankayalapati

Modern rheumatology 28:1004-1008 PubMed29385874

2018

Stage classification of IgG4-related dacryoadenitis and sialadenitis by the serum cytokine environment.

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Motohisa Yamamoto,Kenichi Takano,Ryuta Kamekura,Chisako Suzuki,Shingo Ichimiya,Tetsuo Himi,Hiroshi Nakase,Hiroki Takahashi

View all publications

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Human Interferon alpha 1 ELISA Kit