Human IL-8 ELISA Set (without plates) (ab48481)
製品の概要
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製品名
Human IL-8 ELISA Set (without plates)
IL-8 キット 製品一覧 -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 12.3 pg/ml -
検出範囲
31.25 pg/ml - 1000 pg/ml -
全工程の試験時間
2h 15m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-8 Human in vitro ELISA Set is designed for the quantitative measurement of IL-8 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The IL-8 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA set is intended to measure human IL-8 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Sensitivity: < 29 pg / ml
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-IL-8 antibody 1 vial 2 vials 3 vials IL-8 Capture antibody 1 x 100µl 2 x 100µl 3 x 100µl IL-8 Standard Yellow 5 vials 10 vials 15 vials Ready-to-use TMB 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
研究分野
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機能
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
細胞内局在
Secreted. - Information by UniProt
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別名
- (Ala-IL-8)77
- (Ser-IL-8)72
- 9E3
see all -
参照データベース
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab48481の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Human IL-8 ELISA SetIL-8 Standard curve ranging from 62.5 to 2000 pg/mL
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab48481 は 3 報の論文で使用されています。
- Zhang X et al. Cancer-associated fibroblast-induced lncRNA UPK1A-AS1 confers platinum resistance in pancreatic cancer via efficient double-strand break repair. Oncogene 41:2372-2389 (2022). PubMed: 35264742
- Mollarasouli F et al. Ultrasensitive determination of receptor tyrosine kinase with a label-free electrochemical immunosensor using graphene quantum dots-modified screen-printed electrodes. Anal Chim Acta 1011:28-34 (2018). PubMed: 29475482
- Liang CL et al. Protection Effect of Zhen-Wu-Tang on Adriamycin-Induced Nephrotic Syndrome via Inhibiting Oxidative Lesions and Inflammation Damage. Evid Based Complement Alternat Med 2014:131604 (2014). ELISA . PubMed: 24812565