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AB325443

Human IL-8 ELISA Kit, Chemiluminescent

Human IL-8 ELISA Kit, Chemiluminescent

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Human IL-8 ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Human IL-8 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :1.3 - 940 pg/mL with a sensitivity of 0.47 pg/mL
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1

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Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)

Interpolated concentration of native IL-8 was measured in duplicate at different sample concentrations. Undiluted samples are 100% human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)

Standard curve comparison between the Chemiluminescent Human IL-8 SimpleStep ELISA and the colorimetric Human IL-8 SimpleStep ELISA (ab214030) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)

Dynamic range comparison between the Chemiluminescent Human IL-8 SimpleStep ELISA, colorimetric Human IL-8 SimpleStep ELISA (ab214030), and leading chemiluminescent competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity with a shorter assay time (55 min vs 5 hr for the leading competitor).

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)

Serum of ten individual healthy human male donors and one individual with Alzheimer’s disease (AD) were measured in duplicate. Interpolated values are plotted (mean +/- SD, n=2). For the ten donors, the mean IL-8 concentration was determined to be 30.6 pg/mL with a range of 2.2 – 127.6 pg/mL. The mean IL-8 concentration measured in Alzheimer’s serum was determined to be 4.6 pg/mL.

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)

Interpolated concentration of native IL-8 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 400 unstimulated and stimulated PBMC cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

検出方法

Luminescent

サンプルタイプ

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Cerebral Spinal Fluid

交差種

Human

アッセイタイプ

Sandwich (quantitative)

検出感度

= 0.47 pg/mL

検出範囲

1.3 - 940 pg/mL

アッセイ時間

55m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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製品の詳細

Human IL-8 ELISA Kit, Chemiluminescent (ab325443) is a rapid single-wash sandwich 55-min ELISA to measure Human IL-8 in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant, Cerebrospinal Fluid with a sensitivity of 0.47 pg/ml.

How the assay works

Human IL-8 ELISA Kit, Chemiluminescent (ab325443) SimpleStep ELISA®  employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Please refer to our protocol booklet for more details.

Human IL-8 (ab325443) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-8 antibody [EPR19358-37] BSA and Azide free (Capture) ab242515

- Anti-IL-8 antibody [EPR19358-108] BSA and Azide free (Detector) ab242983

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精度

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.8" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.5" } ]
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回収率

[ { "sample": "Serum", "range": "93 - 103 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "84 - 92 %", "average": "= 88" }, { "sample": "EDTA Plasma", "range": "83 - 90 %", "average": "= 86" }, { "sample": "Heparin Plasma", "range": "82 - 91 %", "average": "= 87" }, { "sample": "Cell culture media", "range": "98 - 104 %", "average": "= 101" }, { "sample": "Cerebral Spinal Fluid", "range": "84 - 94 %", "average": "= 89" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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製品プロトコール

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ターゲットの情報

Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed : 18692776, PubMed : 7636208). Also plays an important role in neutrophil activation (PubMed : 2145175, PubMed : 9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed : 1840701, PubMed : 1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed : 11971003, PubMed : 8662698).
See full target information CXCL8
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websiteProtocolBooklet
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