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AB214030

Human IL-8 ELISA Kit

Human IL-8 ELISA Kit

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(2 Reviews)

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(34 Publications)

Human IL-8 ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human interleukin-8 (IL-8) with a sensitivity of 1.8 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA - 450 nm readout; compatible with any standard plate reader
- Available in different formats to meet various throughput needs, including 384-well plates for higher throughput
- Broad sample compatibility – suitable for serum, plasma, cell culture supernatants, and cerebrospinal fluid
- Cited in over 30 publications

別名を表示する

IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1

12 Images
Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Example of human IL-8 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Example of human IL-8 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Interpolated concentration of native IL-8 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 0.25% stimulated PBMC supernatant. The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-8 concentration was determined to be 61.6 pg/mL with a range of 62 – 174.9 pg/mL; two individuals (Donor #3 and Donor #9) measured below the minimal detectable dose.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Human IL-8 standard curve comparison data.

Standard curve comparison between human IL-8 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA® kit shows a 3-fold increase in sensitivity.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Human IL-8 concentration was interpolated from the standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-8 ELISA kit (ab214030). Wild-type PC-3 cells and CXCL8 knockout PC-3 cells (ab273743) were assessed in duplicate (n=2) and were either treated with 2 μg/ml LPS for 6 hours to induce expression of IL-8 or not treated with LPS. Data are represented as the mean and error bars represent standard deviation.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Recombinant IL-8 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Interpolated concentrations of native IL-8 in human serum.

The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-8 concentration was determined to be 63.2 pg/mL in serum.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Interpolated concentrations of native IL-8 in human PBMC cell culture supernatant.

The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated 1 : 400 and stimulated 1 : 400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Measured values were interpolated from the IL-8 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed. The mean IL-8 concentration was determined to be 2.2 ng/mL in unstimulated, 119.7ng/mL in stimulated, and undetectable in media.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native IL-8 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Example of human IL-8 standard curve in Sample Diluent NS.

The IL-8 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich (quantitative)

検出感度

= 1.8 pg/mL

検出範囲

3.91 - 250 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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下記製品にもご興味をお持ちいただけるかもしれません:

AB241839

Human/Monkey IL-8 Antibody Pair - BSA and Azide free

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0 Reviews

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製品の詳細

Human IL-8 ELISA Kit ab214030 is a rapid single-wash 90-min ELISA kit to measure Human IL-8 in cerebral spinal fluid, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 1.8 pg/mL.

How the assay works
Human IL-8 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit recognizes both native and recombinant human IL-8 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.

Human IL-8 ELISA Kit ab214030 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human IL-8 ELISA Kit ab214030
Human IL-8 ELISA Kit ab214030 has been used to assess inflammation in different physio-pathological contexts such as traumatic brain injury(1), pulmonary disease(2), and osteoarthritis(3).

References:
(1)Jindan Gao et al. 2022, PMID: 36247290,
(2)Rui Zheng et al. 2022, PMID: 35191419,
(3)Zhi Lv et al. 2021, PMID: 34082824

Save your precious samples
Our 384-well Human IL-8 ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human/Monkey IL-8 Antibody Pair - BSA and Azide free ab241839
- Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) ab242515
- Anti-IL-8 antibody [EPR19358-108] - BSA and Azide free (Detector) ab242983

Related and recommended products
Human IL-8 ELISA Kit ab214030 is commonly used to measure IL-8 as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:
- Human TNF alpha ELISA kit ab181421
- Human IL-6 ELISA kit ab178013
- Human IL-1 beta ELISA kit ab214025

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

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精度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" } ]
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回収率

[ { "sample": "Cell culture media", "range": "98 - 104 %", "average": "= 101" }, { "sample": "Cerebral Spinal Fluid", "range": "84 - 94 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "83 - 90 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "84 - 92 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "82 - 91 %", "average": "= 87" }, { "sample": "Serum", "range": "93 - 103 %", "average": "= 99" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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製品プロトコール

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ターゲットの情報

Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed : 18692776, PubMed : 7636208). Also plays an important role in neutrophil activation (PubMed : 2145175, PubMed : 9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed : 1840701, PubMed : 1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed : 11971003, PubMed : 8662698).
See full target information CXCL8
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文献 (34)

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