Human IL-7 ELISA Kit (ab46031)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 6.25 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human IL-7 ELISA Kit
IL-7 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Sample A 8 198.7pg/ml 1.667 0.83% Sample B 8 28.69pg/ml 1.118 3.86% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Sample A 29 198.9pg/ml 3.772 1.89% Sample B 29 25.68pg/ml 1.505 5.84% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 3 pg/ml -
検出範囲
6.25 pg/ml - 200 pg/ml -
添加回収試験
97.6 %
-
全工程の試験時間
2h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-7 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-7 in Human serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific for IL-7 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-7 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-7 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-7 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-7.
Get higher sensitivity in only 90 minutes with Human IL-7 ELISA Kit (ab185984) from our SimpleStep ELISA® range.
-
アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-IL-7 Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 23ml HRP Diluent Red 1 x 12ml 1 x 23ml IL-7 Control Silver 2 vials 4 vials IL-7 Microplate (12 x 8 well strip) 1 unit 2 units IL-7 standard Yellow 2 vials 4 vials Standard Diluent (Serum) 1 x 7ml 2 x 7ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究分野
-
関連性
IL-7 is a cytokine important for B and T cell development. It and the hepatocyte growth factor form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL-7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. It can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that IL-7 plays an essential role in lymphoid cell survival. -
細胞内局在
Secreted -
別名
- IL 7
- IL7
- IL7 nirs variant 6
see all -
参照データベース
- Entrez Gene: 3574 Human
- SwissProt: P13232 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46031の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
-
Typical Standard Curve
Representative Standard Curve using ab46031
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab46031 は 1 報の論文で使用されています。
- Ruan S et al. Treatment with Interleukin-7 Restores Host Defense against Pneumocystis in CD4+ T-Lymphocyte-Depleted Mice. Infect Immun 84:108-19 (2016). PubMed: 26483405