Human IL-5 ELISA Set (without plates) (ab47356)
製品の概要
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製品名
Human IL-5 ELISA Set (without plates) -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 5 pg/ml -
検出範囲
7.8 pg/ml - 250 pg/ml -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-5 Human in vitro ELISA Set is designed for the quantitative measurement of IL-5 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA Set is intended to measure human IL-5 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
These reagents are intended for research use only, not for diagnostic purposes.
The IL-5 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human IL-5.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 10 x 96 tests 5 x 96 tests 15 x 96 tests Chromogen TMB Substrate Solution 4 vials 2 vials 6 vials Detection biotinylated anti-IL-5 antibody 2 x 0.55ml 1 x 0.55ml 3 x 0.55ml IL-5 Capture antibody 2 x 0.5ml 1 x 0.5ml 3 x 0.5ml IL-5 standard Yellow 10 vials 5 vials 15 vials Streptavidin-HRP 2 vials 1 vial 3 vials -
研究分野
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関連性
Interleukin 5 (IL-5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70% amino acid sequence homology. -
細胞内局在
Secreted -
別名
- B cell differentiation factor I
- Eosinophil differentiation factor
- IL5
see all -
参照データベース
- Entrez Gene: 3567 Human
- SwissProt: P05113 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47356の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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IL-5 Human ELISA Set (ab47356)IL-5 Standard curve ranging from 7.8 to 250 pg/mL
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab47356 は論文での使用が確認できていません。