Human IL-4 ELISA Kit (ab46058)
Key features and details
- Sensitivity: 0.7 pg/ml
- Range: 1.1 pg/ml - 35 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-4 ELISA Kit
IL-4 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% sample 6 3.5% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% sample 6 7.3% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 0.7 pg/ml -
検出範囲
1.1 pg/ml - 35 pg/ml -
添加回収試験
107 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 100 75% - 121% -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-4 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in supernatants, buffered solutions, serum and plasma samples.
A monoclonal antibody specific IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL4 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-4.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated anti IL-4 1 x 400µl 2 x 400µl Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml Control 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml IL-4 Microplate (12 x 8 well strips) 1 unit 2 units IL-4 Standard (Lyophilized) 2 vials 4 vials Standard Diluent (Serum) 1 x 7ml 2 x 7ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究分野
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関連性
IL-4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL-4. IL-4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL-4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL-4 encoding distinct isoforms have been reported. -
細胞内局在
Secreted -
別名
- B cell growth factor 1
- B cell stimulatory factor 1
- BCGF 1
see all -
参照データベース
- Entrez Gene: 3565 Human
- Omim: 147780 Human
- SwissProt: P05112 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46058の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical data
Representative standard curve using ab46058
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab46058 は論文での使用が確認できていません。