Human IL-2 Receptor alpha ELISA Kit

製品の詳細

Human IL-2 Receptor alpha ELISA kit (ab272199) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-2 Receptor alpha protein in human serum, plasma and cell culturesupernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-2 Receptor alpha with 1.12 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human IL-2 Receptor alpha is a 55 kDa transmembrane glycoprotein. The protein standard for this ELISA was designed on the extracellular fragment of the IL-2 Receptor alpha glycoprotein. IL-2 Receptor alpha is a receptor for interleukin-2 (IL-2) and is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. Human IL-2 Receptor alpha shares 57.7%, 58.4%, 51.6%, and 91.3% sequence homology with mouse, rat, cow, and monkey, respectively.