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Human IL-2 ELISA Kit High Sensitivity (ab46054)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.97 pg/ml
    • Range: 1.87 pg/ml - 60 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    製品の概要

    • 製品名

      Human IL-2 ELISA Kit High Sensitivity
      IL-2 キット 製品一覧

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      Overall 5 4.2%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      Overall 5 9%

    • サンプルの種類

      Cell culture supernatant, Serum, Plasma

    • アッセイタイプ

      Sandwich (quantitative)

    • 検出感度

      < 0.97 pg/ml

    • 検出範囲

      1.87 pg/ml - 60 pg/ml

    • 添加回収試験

      100 %

      特定サンプルでの回収試験
      サンプルの種類 平均 % 測定範囲
      Cell culture supernatant 95 % - %
      Serum 100 % - %

    • 全工程の試験時間

      2h 00m

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Human

    • 製品の概要

      Abcam’s Human High Sensitivity IL-2 ELISA is to be used for the in-vitro quantitative determination interleukin-2 (IL-2) in Human sera, plasmas, buffered solutions or cell culture media.


      A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.


      This kit will recognize both endogenous and recombinant Human IL-2.


      Get results in 90 minutes with Human IL-2 ELISA Kit (ab174444) from our SimpleStep ELISA® range.

    • アプリケーション

      適用あり: Sandwich ELISAmore details

    • 試験プラットフォーム

      Microplate

    製品の特性

    • 保存方法

      Store at +4°C. Please refer to protocols.
    • 内容 ラベル 1 x 96 tests 1 x 96 tests
      10X Standard Diluent Buffer Black 1 x 15ml 1 x 15ml
      200X Wash Buffer White 1 x 10ml 1 x 10ml
      Amplification Diluent Brown & blue spot 1 x 15ml 1 x 15ml
      Amplifier Yellow 1 x 200µl 1 x 200µl
      Biotinylated anti IL-2 Red 1 x 400µl 1 x 400µl
      Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 7.5ml
      Chromogen TMB Substrate Solution 1 x 11ml 1 x 11ml
      HRP Diluent Red 1 x 23ml 1 x 23ml
      IL-2 Microplate (12 x 8 well strips) 1 unit 1 unit
      IL-2 Standard (Lyophilized) Yellow 2 vials 2 vials
      Standard Diluent (Serum) 1 x 7ml 1 x 7ml
      Stop Reagent Black 1 x 11ml 1 x 11ml
      Streptavidin-HRP 2 x 5µl 2 x 5µl

    • 研究分野

    • 機能

      Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

    • 関連疾患

      Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.

    • 配列類似性

      Belongs to the IL-2 family.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 別名

      • Aldesleukin
      • IL 2
      • IL-2
      • IL2
      • IL2_HUMAN
      • Interleukin 2
      • Interleukin-2
      • interleukin2
      • Involved in regulation of T cell clonal expansion
      • Lymphokine
      • OTTHUMP00000164090
      • POIL2
      • T Cell Growth Factor
      • T-cell growth factor
      • TCGF
      see all

    • 参照データベース

      アプリケーション

      The Abpromise guarantee

      Abpromise保証は、 次のテスト済みアプリケーションにおけるab46054の使用に適用されます

      アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

      アプリケーション Abreviews 特記事項
      Sandwich ELISA
      Use at an assay dependent concentration.
      特記事項
      Sandwich ELISA
      Use at an assay dependent concentration.

      画像

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab46054

      参考文献 (3)

      ab46054 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab46054 は 3 報の論文で使用されています。

      • Zhang L  et al. c-Myb facilitates immune escape of esophageal adenocarcinoma cells through the miR-145-5p/SPOP/PD-L1 axis. Clin Transl Med 11:e464 (2021). PubMed: 34586738
      • Solocinski K  et al. Overcoming hypoxia-induced functional suppression of NK cells. J Immunother Cancer 8:N/A (2020). PubMed: 32345623
      • Li G  et al. CRP, IL-2 and TNF-a level in patients with uremia receiving hemodialysis. Mol Med Rep 17:3350-3355 (2018). PubMed: 29257244

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