Human IL-2 ELISA Kit High Sensitivity (ab46054)
Key features and details
- Sensitivity: 0.97 pg/ml
- Range: 1.87 pg/ml - 60 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-2 ELISA Kit High Sensitivity
IL-2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5 4.2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 5 9% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 0.97 pg/ml -
検出範囲
1.87 pg/ml - 60 pg/ml -
添加回収試験
100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 95 % - % Serum 100 % - % -
全工程の試験時間
2h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human High Sensitivity IL-2 ELISA is to be used for the in-vitro quantitative determination interleukin-2 (IL-2) in Human sera, plasmas, buffered solutions or cell culture media.
A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-2.
Get results in 90 minutes with Human IL-2 ELISA Kit (ab174444) from our SimpleStep ELISA® range.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 200X Wash Buffer White 1 x 10ml Amplification Diluent Brown & blue spot 1 x 15ml Amplifier Yellow 1 x 200µl Biotinylated anti IL-2 Red 1 x 400µl Biotinylated Antibody Diluent Red 1 x 7.5ml Chromogen TMB Substrate Solution 1 x 11ml HRP Diluent Red 1 x 23ml IL-2 Microplate (12 x 8 well strips) 1 unit IL-2 Standard (Lyophilized) Yellow 2 vials Standard Diluent (Serum) 1 x 7ml Stop Reagent Black 1 x 11ml Streptavidin-HRP 2 x 5µl -
研究分野
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機能
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
関連疾患
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
配列類似性
Belongs to the IL-2 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Aldesleukin
- IL 2
- IL-2
see all -
参照データベース
- Entrez Gene: 3558 Human
- Omim: 147680 Human
- SwissProt: P60568 Human
- Unigene: 89679 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46054の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative standard curve using ab46054
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab46054 は 3 報の論文で使用されています。
- Zhang L et al. c-Myb facilitates immune escape of esophageal adenocarcinoma cells through the miR-145-5p/SPOP/PD-L1 axis. Clin Transl Med 11:e464 (2021). PubMed: 34586738
- Solocinski K et al. Overcoming hypoxia-induced functional suppression of NK cells. J Immunother Cancer 8:N/A (2020). PubMed: 32345623
- Li G et al. CRP, IL-2 and TNF-a level in patients with uremia receiving hemodialysis. Mol Med Rep 17:3350-3355 (2018). PubMed: 29257244