Human IL-17a ELISA Kit (ab83688)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 3.125 pg/ml - 100 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-17a ELISA Kit
IL-17A キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 6 3.3% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 6 5.2% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 3 pg/ml -
検出範囲
3.125 pg/ml - 100 pg/ml -
添加回収試験
105 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 121 113% - 130% -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-17a Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative of IL-17a in supernatants, buffered solutions, serum, plasma and other body fluids.
A monoclonal antibody specific for IL-17a has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-17a concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-17a are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-17a present in the samples.
This kit will recognize both endogenous and recombinant Human IL-17a.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-IL17a Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 12ml 1 x 23ml IL-17a Microplate (12 x 8 well strips) 1 unit 2 units IL-17a Standard (Lyophilized) Orange 2 vials 4 vials Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究分野
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機能
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
組織特異性
Restricted to activated memory T-cells. -
配列類似性
Belongs to the IL-17 family. -
翻訳後修飾
Found both in glycosylated and nonglycosylated forms. -
細胞内局在
Secreted. - Information by UniProt
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別名
- CTLA 8
- CTLA-8
- CTLA8
see all -
参照データベース
- Entrez Gene: 3605 Human
- Omim: 603149 Human
- SwissProt: Q16552 Human
- Unigene: 41724 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab83688の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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ELISA: IL-17 Human ELISA Kit (ab83688)
IL-17 measured in cell culture supernatants (dilution range 1/1-1/100) from control (PBMC) or treated ((PBMC+PHA) 48 hours; 2% PHA-M; LifeTechnologies) cells (duplicates +/- SD).
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Typical Standard CurveRepresentative Standard Curve using ab83688
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab83688 は 6 報の論文で使用されています。
- Liu B et al. Reciprocal regulation of interleukin-17A and interleukin-22 secretion through aryl hydrocarbon receptor activation in CD4+ T cells of patients with vitiligo. Exp Ther Med 21:158 (2021). PubMed: 33456525
- Caselli G et al. Pharmacological characterisation of CR6086, a potent prostaglandin E2receptor 4 antagonist, as a new potential disease-modifying anti-rheumatic drug. Arthritis Res Ther 20:39 (2018). PubMed: 29490676
- Huang G et al. Platelet-rich plasma shows beneficial effects for patients with knee osteoarthritis by suppressing inflammatory factors. Exp Ther Med 15:3096-3102 (2018). PubMed: 29599843
- Zhang Z et al. Astragalus mongholicus (Fisch.) Bge Improves Peripheral Treg Cell Immunity Imbalance in the Children With Viral Myocarditis by Reducing the Levels of miR-146b and miR-155. Front Pediatr 6:139 (2018). PubMed: 29977885
- Liu XG et al. Expression levels of IL-17 and TNF-a in degenerated lumbar intervertebral discs and their correlation. Exp Ther Med 11:2333-2340 (2016). PubMed: 27284317
- Yu X et al. Intensive Cytokine induction in Pandemic H1N1 Influenza Virus Infection Accompanied by Robust Production of IL-10 and IL-6. PLoS One 6:e28680 (2011). ELISA . PubMed: 22174866