Human IL-13 Receptor alpha ELISA Kit (ab46066)
Key features and details
- Sensitivity: 150 pg/ml
- Range: 156 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-13 Receptor alpha ELISA Kit
IL-13 receptor alpha 2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% A 14 3024pg/ml 93.67 3.1% B 14 1054pg/ml 29.69 2.82% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% A 8 2744pg/ml 90.1 3.28% B 8 725pg/ml 43.9 6.06% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 150 pg/ml -
検出範囲
156 pg/ml - 5000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum = 90 79% - 100% -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-13 Receptor alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interleukin 13 Receptor (IL-13 Receptor alpha, also known as CD213 alpha 2) in Human serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific for IL-13 Receptor alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-13 Receptor alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-13 Receptor alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-13 Receptor alpha present in the samples.
This kit will recognize both endogenous and recombinant Human IL-13 Receptor alpha.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-IL-13 Receptor alpha Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 12ml 1 x 23ml IL-13 Receptor alpha Microplate (12 x 8 well strips) 1 unit 2 units IL-13 Receptor alpha Standard (Lyophilized) Yellow 2 vials 4 vials Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究分野
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機能
Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4). -
配列類似性
Belongs to the type I cytokine receptor family. Type 5 subfamily.
Contains 1 fibronectin type-III domain. -
ドメイン
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
細胞内局在
Membrane. - Information by UniProt
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別名
- Cancer/testis antigen 19
- CD213a2
- CT19
see all -
参照データベース
- Entrez Gene: 3598 Human
- Omim: 300130 Human
- SwissProt: Q14627 Human
- Unigene: 336046 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46066の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab46066
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab46066 は論文での使用が確認できていません。