AB46066

Human IL-13 Receptor alpha ELISA Kit

Name: Human IL-13 Receptor alpha ELISA Kit (ab46066) | Abcam
Brand: Abcam Limited
SKU: AB46066
Price: 139000 JPY
Availability: InStock

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Human IL-13 Receptor alpha ELISA Kit is a Sandwich ELISA for the measurement of Human IL-13 Receptor alpha in Human in Biofluids, Cell Culture Media samples.

別名を表示する

CD213a2, IL13R, IL13RA2, Interleukin-13 receptor subunit alpha-2, IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, IL-13R-alpha-2, IL-13RA2, Interleukin-13-binding protein

1 Images

Sandwich ELISA - Human IL-13 Receptor alpha ELISA Kit (AB46066)

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 Receptor alpha ELISA Kit (AB46066)

Representative Standard Curve using ab46066

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma, Cell culture supernatant, Serum

交差種

Human

アッセイタイプ

Sandwich

検出感度

< 150 pg/mL

検出範囲

156 - 5000 pg/mL

アッセイ時間

3h 45m

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Abcam's IL-13 Receptor alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interleukin 13 Receptor (IL-13 Receptor alpha, also known as CD213 alpha 2) in Human serum, plasma, buffered solutions and cell culture medium.

A monoclonal antibody specific for IL-13 Receptor alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-13 Receptor alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-13 Receptor alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-13 Receptor alpha present in the samples.

This kit will recognize both endogenous and recombinant Human IL-13 Receptor alpha.

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精度

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 8, "mean": "2744 pg/mL", "standardDeviation": "90.1", "coefficientOfVariability": "3.28" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 8, "mean": "725 pg/mL", "standardDeviation": "43.9", "coefficientOfVariability": "6.06" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 14, "mean": "3024 pg/mL", "standardDeviation": "93.67", "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 14, "mean": "1054 pg/mL", "standardDeviation": "29.69", "coefficientOfVariability": "2.82" } ]

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回収率

[ { "sample": "Serum", "range": "79 - 100 %", "average": "= 90" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46066-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46066-CMP03" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46066-CMP01" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46066-CMP02" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46066-CMP04" }, { "size":"2 x 1 Vial", "name":"IL-13 Receptor alpha Standard (Lyophilized)", "number":"AB46066-CMP09" }, { "size":"1 x 1 Unit", "name":"IL-13 Receptor alpha Microplate (12 x 8 well strips)", "number":"AB46066-CMP08" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-IL-13 Receptor alpha", "number":"AB46066-CMP07" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46066-CMP10" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46066-CMP06" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46066-CMP05" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46066-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46066-CMP03", "productcode":"" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-IL-13 Receptor alpha", "number":"AB46066-CMP07", "productcode":"" }, { "size":"4 x 1 Vial", "name":"IL-13 Receptor alpha Standard (Lyophilized)", "number":"AB46066-CMP09", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46066-CMP05", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46066-CMP10", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46066-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46066-CMP06", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46066-CMP04", "productcode":"" }, { "size":"2 x 1 Unit", "name":"IL-13 Receptor alpha Microplate (12 x 8 well strips)", "number":"AB46066-CMP08", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46066-CMP01", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Cell surface receptor that plays a role in the regulation of IL-13-mediated responses (PubMed : 11861389, PubMed : 17030238). Functions as a decoy receptor that inhibits IL-13- and IL-4-mediated signal transduction via the JAK-STAT pathway and thereby modulates immune responses and inflammation (PubMed : 11861389, PubMed : 17030238). Serves as a functional signaling receptor for IL-13 in an alternative pathway involving AP-1 ultimately leading to the production of TGFB1 (PubMed : 16327802).

See full target information IL13RA2

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websiteProtocolBooklet

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Abcam product promise

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Human IL-13 Receptor alpha ELISA Kit