AB303763

Human IL-13 receptor alpha 1 ELISA Kit

Recombinant
SimpleStep
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Human IL-13 receptor alpha 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-13 receptor alpha 1 with a sensitivity of 5.939 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

CD213a1, IL13R, IL13RA, IL13RA1, Interleukin-13 receptor subunit alpha-1, IL-13 receptor subunit alpha-1, IL-13R subunit alpha-1, IL-13R-alpha-1, IL-13RA1, Cancer/testis antigen 19, CT19

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Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Interpolated concentrations of human IL-13 receptor alpha 1 in serum, plasma – citrate, plasma – EDTA, and plasma – heparin.

Interpolated concentration of native IL-13 receptor alpha 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1 : 16 serum, 1 : 16 plasma – citrate, 1 : 16 plasma – EDTA, and 1 : 16 plasma – heparin. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Interpolated concentrations of human IL-13 receptor alpha 1 in HepG2 cell culture supernatant.

Interpolated concentration of native IL-13 receptor alpha 1 was measured in duplicate at different sample concentrations. Undiluted samples are 100% for HepG2 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Example of human IL-13 receptor alpha 1 standard curve in Sample Diluent NS.

Example of human IL-13 receptor alpha 1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Interpolated concentrations of IL-13 receptor alpha 1 in normal human serum donors.

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean IL-13 receptor alpha 1 concentration was determined to be 23.8 ng/mL with a range of 15.3 – 35.4 ng/mL

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cell culture supernatant

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 5.939 pg/mL

検出範囲

31.25 - 2000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human IL-13 receptor alpha 1 SimpleStep ELISA® kit (ab303763) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-13 receptor alpha 1 protein in serum, plasma (cit), plasma (hep), plasma (EDTA) and cell culture supernatant. Quantitate Human IL-13 receptor alpha 1 with 5.939 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "109 - 117 %", "average": "= 114" }, { "sample": "Citrate plasma", "range": "84 - 100 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "93 - 110 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "82 - 113 %", "average": "= 100" }, { "sample": "Serum", "range": "90 - 113 %", "average": "= 105" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.

See full target information IL13RA1

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websiteProtocolBooklet

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Abcam product promise

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Human IL-13 receptor alpha 1 ELISA Kit