Human IL-12 p40 + IL-12 p70 ELISA Kit (ab46037)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 62.5 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-12 p40 + IL-12 p70 ELISA Kit
IL-12B キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 3.7% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Sample 1 18 2277pg/ml 160 7% Sample 2 18 1199pg/ml 108 9% Sample 3 18 479pg/ml 49 10.3% Sample 4 18 1488pg/ml 123 8.3% Sample 5 18 826pg/ml 106 12.8% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 20 pg/ml -
検出範囲
62.5 pg/ml - 2000 pg/ml -
添加回収試験
100 %
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全工程の試験時間
1h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-12 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-12 in Human serum, plasma, buffered solutions and supernatants.
A monoclonal antibody specific for IL-12 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-12 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-12 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-12 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-12, and the Human Il-12 p40 monomer, dimer or heterodimer p70 (p40 + p35) forms of the molecule.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml Biotinylated anti-IL12 Red 2 x 0.4ml 1 x 0.4ml Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml HRP Diluent Red 1 x 23ml 1 x 12ml IL-12 Control Silver 4 vials 2 vials IL-12 Microplate (12 x 8 well strips) 2 units 1 unit IL-12 Standard (Lyophilized) Yellow 4 vials 2 vials Plastic Plate Covers 4 units 2 units Standard Diluent (Serum) 2 x 7ml 1 x 7ml Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
研究分野
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機能
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
関連疾患
Mendelian susceptibility to mycobacterial disease
Psoriasis 11 -
配列類似性
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
翻訳後修飾
Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified. -
細胞内局在
Secreted. - Information by UniProt
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別名
- CLMF
- CLMF p40
- CLMF2
see all -
参照データベース
- Entrez Gene: 3593 Human
- Omim: 161561 Human
- SwissProt: P29460 Human
- Unigene: 674 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46037の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab46037
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab46037 は 1 報の論文で使用されています。
- Gu Y et al. Enhancing Fatty Acid Catabolism of Macrophages Within Aberrant Breast Cancer Tumor Microenvironment Can Re-establish Antitumor Function. Front Cell Dev Biol 9:665869 (2021). PubMed: 33937269