Human IL-10 ELISA Kit High Sensitivity (ab46059)

Thank you for contacting us.

I am sorry about the continued problems that you have been experiencing using this kit. Unfortunately I do not have any further suggestions to help with this issue. I have created a credit note/refund for the original purchase. Your credit note ID is 20001.

I am sorry that this product did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

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The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

I would like to suggest that you try using another kit to determine whether this is a product specific issue. If you provide me with the orginal Abcam order confirmation code I can send you a free of charge replacement for this product.



Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

I've passed this information on to the lab for you.

They feel that the data enclosed are pretty good, except the blank which is indeed a little bit high, and which should rather be considered as a zero. They also also would like to confirm what you use as Blank/zero?

Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Thank you for that additional information. The matrix effect could be called quenching. The high protein concentration of serum samples or components contained in samples can affect assay response to the analyte (IL-10) and give background. If you dilute samples, then the matrix is altered and the recovery of analyte is better. The results obtained should be multiplied by the dilution factor.There is two different diluents provided in the kit, depending of what kind of samples are assaying (serum/plasmas or cell supernatents). We do recommend using these. I hope this information is helpful to you. Please do not hesitate to contact us should you have any questions.

Thank you for contacting Abcam.

I have had the opportunity to speak with the lab regarding this issue. The low signal obtained may be linked to the use of human serum as standard curve diluent. Have you diluted the standards in human serum or in standard diluent buffer PBS/BSA? They also advise diluting the samples 1:2 to minimize the matrix effect.

Please let me know if you have any questions.

Thank you for your call today, and for letting us know about the trouble with this kit. As we discussed, please forward any data you have using this kit, and I will see if there are any suggestions that we can make to improve the results. I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

Thank you for your call yesterday and for your patience while i have been in touch with the lab regarding these IL10 kits. Here is the reply that I received from my contact at the lab: "To be on the safe side regarding sensitivity, my opinion would be to use the High Sensitivity format, with dilution factor for plasmas 1:2 or 1:5. On the other hand, for someone new to ELISA, the Non-High Sensitivity one is easier to assay (less steps & more robust). According to general knowledge, IL-10 concentration is increased in HIV positive plasmas, so the detection level should be convenient." I hope this information will be useful in determining which of these kits will be most suitable for your studies, but please let me know if you have any further questions or if there is anything else that we can do for you.     v\:* {behavior:url(#default#VML);} o\:* {behavior:url(#default#VML);} w\:* {behavior:url(#default#VML);} .shape {behavior:url(#default#VML);}