Human IL-1 beta ELISA Kit (ab46052)
Key features and details
- Sensitivity: 6.5 pg/ml
- Range: 15.6 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-1 beta ELISA Kit
IL-1 beta キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% 6 4.5% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% 6 8.7% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
6.5 pg/ml -
検出範囲
15.6 pg/ml - 500 pg/ml -
添加回収試験
102.2 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 102.2 15.6pg/ml - 500pg/ml -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human IL-1 beta ab46052 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 beta in Human serum, plasma, buffered solutions or cell culture medium.
A monoclonal antibody specific for IL-1 beta has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-1 beta concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-1 beta are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-1 beta present in the samples.
This kit will recognize both endogenous and recombinant Human IL-1 beta.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 1 x 15ml 200X Wash Buffer White 1 x 10ml 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml 1 x 7.5ml Biotinylated anti-IL-1 beta Red 1 x 400µl 2 x 400µl 1 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml 1 x 11ml Control Silver 2 vials 4 vials 2 vials HRP Diluent Red 1 x 12ml 1 x 23ml 1 x 12ml IL-1 beta Microplate (12 x 8 well strips) 1 unit 2 units 1 unit IL1 beta standard Yellow 2 vials 4 vials 2 vials Standard Diluent (Serum) 1 x 7ml 2 x 7ml 1 x 7ml Stop Reagent Black 1 x 11ml 2 x 11ml 1 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl 2 x 5µl -
研究分野
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機能
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. -
組織特異性
Expressed in activated monocytes/macrophages (at protein level). -
配列類似性
Belongs to the IL-1 family. -
翻訳後修飾
Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. -
細胞内局在
Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. - Information by UniProt
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別名
- Catabolin
- H1
- IFN beta inducing factor
see all -
参照データベース
- Entrez Gene: 3553 Human
- Omim: 147720 Human
- SwissProt: P01584 Human
- Unigene: 126256 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46052の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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ELISA: IL-1 beta Human ELISA Kit (ab46052)
Standard curve of human IL-1β in standard diluent with background signal subtracted (duplicates; +/- SD).
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ELISA: IL-1 beta Human ELISA Kit (ab46052)IL-1β detected in supernatants from control THP-1 cells (-) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) and 1 ug x mL-1 LPS (Sigma) for the last 6 hours (P+L).
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Typical Standard CurveRepresentative Standard Curve using ab46052
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (66)
ab46052 は 66 報の論文で使用されています。
- Hsueh YH et al. Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model. J Orthop Translat 38:141-155 (2023). PubMed: 36381245
- Ji J et al. lncRNA OIP5-AS1 knockdown or miR-223 overexpression can alleviate LPS-induced ALI/ARDS by interfering with miR-223/NLRP3-mediated pyroptosis. J Gene Med 24:e3385 (2022). PubMed: 34346534
- Ye T et al. LncRNA CCAT1 is overexpressed in tuberculosis patients and predicts their survival. Immun Inflamm Dis 10:218-224 (2022). PubMed: 34847295
- Cansby E et al. Silencing of STE20-type kinase STK25 in human aortic endothelial and smooth muscle cells is atheroprotective. Commun Biol 5:379 (2022). PubMed: 35440683
- Chen W et al. The Molecular Mechanism of Long Non-Coding RNA MALAT1-Mediated Regulation of Chondrocyte Pyroptosis in Ankylosing Spondylitis. Mol Cells 45:365-375 (2022). PubMed: 35680372