AB46052

Human IL-1 beta ELISA Kit

Name: Human IL-1 beta ELISA Kit (ab46052) | Abcam
Brand: Abcam Limited
SKU: AB46052
Price: 109000 JPY
Availability: InStock

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(83 Publications)

Human IL-1 beta ELISA Kit is a Sandwich ELISA for the measurement of Human IL-1 beta in Human in Biofluids, Cell Culture Media samples.

別名を表示する

IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin

3 Images

ELISA - Human IL-1 beta ELISA Kit (AB46052)

ELISA

Lab

ELISA - Human IL-1 beta ELISA Kit (AB46052)

Standard curve of human IL-1β in standard diluent with background signal subtracted (duplicates; +/- SD).

ELISA

Lab

ELISA - Human IL-1 beta ELISA Kit (AB46052)

IL-1β detected in supernatants from control THP-1 cells (-) or cells stimulated for 24 hours with 50 ng x mL^-1 of PMA (ab120297) and 1 ug x mL^-1 LPS (Sigma) for the last 6 hours (P+L).

sELISA

Supplier Data

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB46052)

Representative Standard Curve using ab46052

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma, Cell culture supernatant, Serum

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 6.5 pg/mL

検出範囲

15.6 - 500 pg/mL

アッセイ時間

3h 45m

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Abcam's Human IL-1 beta ab46052 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 beta in Human serum, plasma, buffered solutions or cell culture medium.

A monoclonal antibody specific for IL-1 beta has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-1 beta concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-1 beta are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-1 beta present in the samples.

This kit will recognize both endogenous and recombinant Human IL-1 beta.

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精度

[ { "reproducibilityType": "Inter", "sample": "Sample 6", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.7" }, { "reproducibilityType": "Intra", "sample": "Sample 6", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]

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回収率

[ { "sample": "Serum", "range": "72 - 105 %", "average": "= 89" } ]

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製品内容

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46052-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46052-CMP04" }, { "size":"4 x 1 Vial", "name":"IL1 beta standard", "number":"AB46052-CMP11" }, { "size":"4 x 1 Vial", "name":"Control", "number":"AB46052-CMP09" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46052-CMP02" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46052-CMP07" }, { "size":"2 x 1 Unit", "name":"IL-1 beta Microplate (12 x 8 well strips)", "number":"AB46052-CMP10" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46052-CMP05" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46052-CMP03" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46052-CMP12" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-IL-1 beta", "number":"AB46052-CMP01" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46052-CMP06" }, { "size":"2 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46052-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46052-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46052-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"IL1 beta standard", "number":"AB46052-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Control", "number":"AB46052-CMP09", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46052-CMP05", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46052-CMP02", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-IL-1 beta", "number":"AB46052-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46052-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-1 beta Microplate (12 x 8 well strips)", "number":"AB46052-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46052-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46052-CMP12", "productcode":"" }, { "size":"1 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46052-CMP08", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46052-CMP03", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Potent pro-inflammatory cytokine (PubMed : 10653850, PubMed : 12794819, PubMed : 28331908, PubMed : 3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed : 3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed : 10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed : 12794819). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 33377178, PubMed : 33883744). Acts as a sensor of S.pyogenes infection in skin : cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed : 28331908).

See full target information IL1B

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文献 (83)

Recent publications for all applications. Explore the full list and refine your search

Cardiovascular diabetology 24:203 PubMed40361188

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Diabetes mellitus aggravates myocardial inflammation and oxidative stress in aortic stenosis: a mechanistic link to HFpEF features.

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Melissa Herwig,Marcel Sieme,Andrea Kovács,Muchtiar Khan,Andreas Mügge,Wolfgang E Schmidt,Ferhat Elci,Shan Sasidharan,Peter Haldenwang,Jan Wintrich,Benjamin Sasko,Ibrahim Akin,Máthé Domokos,Francesco Paneni,Ibrahim El-Battrawy,Zoltán V Varga,Francisca Saraiva,Adelino F Leite-Moreira,Péter Ferdinandy,Loek van Heerebeek,Inês Falcão-Pires,Nazha Hamdani

Journal of clinical medicine 14: PubMed40364209

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Assessment of Serum Cytokine Levels in Keratoconus Patients.

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Medicine 104:e41699 PubMed40068086

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Drug development research 86:e70065 PubMed39950329

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Exosomal ALKBH5 Alleviates Vascular Calcification by Suppressing Cell Apoptosis via m6A-Modified GSDME.

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Guian Xu,Qingman Li,Lijie Zhu,Tingjie Yang,Yapan Yang,Honghui Yang

Methods in molecular biology (Clifton, N.J.) 2878:273-291 PubMed39546268

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Methodologies for Mitochondrial Omic Profiling During Spaceflight.

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Activation of Mammary Epithelial and Stromal Fibroblasts upon Exposure to Metabolites.

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Jamilah H Alshehri,Huda K Al-Nasrallah,Mysoon M Al-Ansari,Abdelilah Aboussekhra

Folia neuropathologica 62:156-170 PubMed39165203

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Pediatric pulmonology 59:3371-3382 PubMed39115441

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Cholesterol profiling reveals 7β-hydroxycholesterol as a pathologically relevant peripheral biomarker of Alzheimer's disease.

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Junghee Ha,Go Eun Kwon,Yumi Son,Soo Ah Jang,So Yeon Cho,Soo Jin Park,Hyunjeong Kim,Jimin Lee,Juseok Lee,Dongryul Seo,Myeongjee Lee,Do Yup Lee,Man Ho Choi,Eosu Kim

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DNM3OS/miR-127-5p/CDH11, activates Wnt3a/β-catenin/LEF-1 pathway to form a positive feedback and aggravate spine facet joint osteoarthritis.

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Jing Wang,Zhenyu Yang,Xiuming He,Yeyang Wang,Dixin Luo,Wangyang Xu,Hongtao Zhang,Xiaozhong Zhou

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Human IL-1 beta ELISA Kit