AB100548

Human IgG1 ELISA Kit

(1 Review)

(2 Publications)

Human IgG1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IgG1 in Human in Biofluids, Cell Culture Media samples.

別名を表示する

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

2 Images

Sandwich ELISA - Human IgG1 ELISA Kit (AB100548)

sELISA

Unknown

Sandwich ELISA - Human IgG1 ELISA Kit (AB100548)

Representative standard curve using ab100548

sELISA

Lab

Sandwich ELISA - Human IgG1 ELISA Kit (AB100548)

Human IgG1 measured in biological fluids showing quantity (pg) per mL of tested sample. Human serum and plasma were diluted 1e6 - 64e6 fold. Human saliva and urine were diluted 1e3-64e3 fold.

Key facts

検出方法

Colorimetric

サンプルタイプ

Saliva, Urine, Plasma, Cell culture supernatant, Serum

交差種

Human

アッセイタイプ

Sandwich (quantitative)

検出感度

< 0.05 ng/mL

検出範囲

0.05 - 40 ng/mL

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Abcam's IgG1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IgG1 in serum and plasma.

This assay employs an antibody specific for Human IgG1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IgG1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IgG1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgG1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples

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回収率

[ { "sample": "Serum", "range": "75 - 97 %", "average": "= 87" }, { "sample": "Plasma", "range": "71 - 94 %", "average": "= 81.5" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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製品プロトコール

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ターゲットの情報

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.

See full target information IGHG1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 13: PubMed37189383

2023

Engineering of a Bispecific Nanofitin with Immune Checkpoint Inhibitory Activity Conditioned by the Cross-Arm Binding to EGFR and PDL1.

Applications

Unspecified application

Species

Unspecified reactive species

Perrine Jacquot,Javier Muñoz-Garcia,Maurine Fleury,Denis Cochonneau,Rémi Gaussin,Elise Enouf,Caroline Roze,Emilie Ollivier,Mathieu Cinier,Dominique Heymann

Scientific reports 5:11018 PubMed26154774

2015

Membrane-less microfiltration using inertial microfluidics.

Applications

Unspecified application

Species

Unspecified reactive species

Majid Ebrahimi Warkiani,Andy Kah Ping Tay,Guofeng Guan,Jongyoon Han

View all publications

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websiteProtocolBooklet

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Abcam product promise

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Human IgG1 ELISA Kit