AB195215

Human IgG ELISA Kit

Name: Human IgG ELISA Kit (ab195215) | Abcam
Brand: Abcam Limited
SKU: AB195215
Price: 118000 JPY
Availability: InStock
Rating: 4 (6 reviews)

SimpleStep
詳細を見る

(6 Reviews)

(37 Publications)

Human IgG ELISA kit is a single-wash, 90-minute SimpleStep ELISA^® assay designed for the quantification of human Immunoglobulin G (IgG) with a sensitivity of 0.02 ng/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA - 450nm readout; works on any plate reader
- High-throughput options – available in 96-well and 384-well formats, supporting large-scale sample processing.
-Note: This kit is designed to detect total human IgG across all four isotypes and uses a natively purified human IgG standard. Some variability may be observed due to differences in sample isotype composition and the native standard material. For isotype-specific measurement, isotype-specific kits are listed in the product details section.
- Cited in over 20 publications

別名を表示する

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

14 Images

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

sELISA

Supplier Data

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

IgG levels in individual healthy donors.

Ten individual healthy donors were evaluated for the presence of IgG in serum using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution (1 : 5x10⁶). The mean level of IgG was found at 12.5 mg/mL with a range of 9 – 18.4mg/mL.

sELISA

Lab

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Interpolated concentrations of Human IgG in serum in 96-well vs. 384-well plates

Interpolated concentration of native IgG was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 1,000,000 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Interpolated concentrations of Human IgG in human liver extract in 96-well vs. 384-well plates

Interpolated concentration of native IgG was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100 ng/mL human liver extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Lab

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Example of Human IgG standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Example of Human IgG standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

ELISA

Unknown

ELISA - Human IgG ELISA Kit (AB195215)

IgG1-4 isotypes are detected by this kit.

Human IgG1, IgG2, IgG3 and IgG4 were tested at 5ng/mL

sELISA

Unknown

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Linearity of dilution – native IgG in human serum, plasma (citrate, EDTA and Heparin), and culture media.

Native IgG was measured in the human serum, plasma citrate, plasma EDTA, plasma heparin and culture media in a 2-fold dilution series. Sample dilutions were made in Sample Diluent NS for all samples, purified IgG was spiked into culture media and diluted in a 2-fold dilution series in Sample Diluent NS

sELISA

Unknown

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Raw data values for example of Human IgG standard curve in Sample Diluent NS.

Raw data values are shown in the table

sELISA

Unknown

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Linearity of dilution – native IgG in human milk, urine, saliva and HLH extract.

Native IgG was measured in milk, saliva, urine and liver homogenate (HLH) in a 2-fold dilution series. Sample dilutions were made in Sample Diluent NS for all samples except for HLH, which was carried out in 1X cell extraction buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Comparison of IgG levels in Human milk, urine and saliva.

Bodily fluids from 3 different donors were evaluated for the presence of IgG using this assay. Results were interpolated from the standard curve in sample diluent NS and corrected for sample dilution (1 : 2.5x10⁴). The mean levels in Milk were found at 20.7 μg/mL, in Urine at 0.8 μg/mL and in Saliva at 11.1 μg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Example of Human IgG standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Example of Human IgG standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Raw data values for example of Human IgG standard curve in 1X Cell Extraction Buffer PTR.

Raw data values are shown in the table

sELISA

Lab

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Key facts

検出方法

Colorimetric

サンプルタイプ

Tissue Lysate, Cerebral Spinal Fluid, Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 0.02 ng/mL

検出範囲

0.23 - 15 ng/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human IgG ELISA Kit ab195215 is a rapid single-wash 90-min ELISA kit to measure Human IgG in tissue lysate, cerebral spinal fluid, saliva, urine, cell culture supernatant, milk, serum and plasma. This SimpleStep ELISA® sensitivity is 0.02 ng/mL.

How the assay works

Human IgG SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit is designed to detect total human IgG across all four isotypes and uses a natively purified human IgG standard. Some variability may be observed due to differences in sample isotype composition and the native standard material. For isotype-specific measurement, please use the kits listed below:
- Human IgG1: ab317543
- Human IgG2: ab202402
- Human IgG3: ab318929
- Human IgG4: ab320788

Human IgM, human IgA and human IgE were prepared at 10 ng/mL and 250 ng/mL in Sample Diluent NS and assayed for cross reactivity. No cross-reactivity was observed for IgM or IgE at either concentration with a mean OD deviation from background of -0.01. No cross-reactivity was observed for IgA at 10 ng/mL and only 0.4% cross-reactivity at 250 ng/mL of IgA. Purified human IgG was assayed at 5 ng/mL in the presence and absence of 250 ng/mL of human IgM, human IgA and human IgE to determine interference. After background subtraction, recovery of human IgG was observed at a mean of 98% with a standard deviation of 0.06.

Human IgG ELISA Kit ab195215 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human IgG ELISA Kit ab195215

Human IgG ELISA Kit ab195215 has been used to study human immunoglobulin g in autoimmune diseases and infections in various physio-pathological contexts such as obesity(1)and SARS-CovV-2(2).

References:

⁽¹⁾Leonid Kagan et al. 2023, PMID: 36949370,
⁽²⁾Peter D Sun et al. 2023, PMID: 37821447

Save your precious samples

Our 384-well Human IgG ELISA Kit only requires a maxmum of 12.5µL

Related and recommended products

Human IgG ELISA Kit ab195215 is commonly used to measure immune system functioning.

Other ELISA kits to measure Immunoglobulins include:

- Human IgM ELISA kit ab214568
- Human IgA ELISA kit ab196263
- Human IgD ELISA kit ab320794
- Human IgE ELISA kit ab195216

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "14.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" } ]

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回収率

[ { "sample": "Milk", "range": "83 - 94 %", "average": "= 89" }, { "sample": "Serum", "range": "88 - 125 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "87 - 93 %", "average": "= 90.17" }, { "sample": "Cerebral Spinal Fluid", "range": "88 - 105 %", "average": "= 98" }, { "sample": "Urine", "range": "82 - 93 %", "average": "= 87" }, { "sample": "Saliva", "range": "80 - 106 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "100 - 100 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "98 - 102 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "96 - 115 %", "average": "= 107" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Immunoglobulin G (IgG) serves as an essential component of the immune system functioning mainly as an antibody. It is found in blood and extracellular fluid making it one of the most prevalent antibodies in the human body. IgG weighs approximately 150 kDa and it is expressed by B cells as part of the humoral response to pathogens. Alternate names include gamma globulin and Ig gamma. IgG antibodies form a critical aspect of adaptive immunity by targeting specific antigens for neutralization or destruction.

Biological function summary

IgG participates in several immune processes by binding to antigens and forming immune complexes. It facilitates pathogen opsonization initiating phagocytosis by immune cells like macrophages and neutrophils. IgG also activates the classical complement pathway which enhances pathogen clearance. IgG exists in a monomeric form and can cross the placenta providing passive immunity to the fetus. In laboratory settings its high specificity makes it valuable in techniques like IgG ELISA for detecting antigen presence.

Pathways

IgG plays an integral role in the complement system and the adaptive immune response. It partners with proteins such as C1q to initiate the classical complement pathway amplifying the body's ability to detect and remove pathogens. IgG also interacts with Fc receptors on immune cells modulating immune cell activity and facilitating antigen presentation to T cells. These interactions enhance the immune system's efficiency in recognizing and responding to diverse pathogens.

IgG has connections to autoimmune diseases and chronic infections. Elevated levels of IgG can indicate autoimmune conditions like lupus or rheumatoid arthritis where the immune system mistakenly attacks healthy tissue. During chronic infections such as HIV variations in IgG responses can affect disease progression and treatment efficacy. In these contexts IgG interacts with proteins involved in the immune dysregulation characteristic of such diseases highlighting its significant role in both protection and pathology.

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製品プロトコール

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ターゲットの情報

Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region) (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain. Each Ig molecule has two antigen-binding sites with remarkable affinity for a particular antigen due to V-(D)-J rearrangement, somatic hypermutations and affinity maturation of the variable domains upon antigen exposure (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region defines the Ig isotype that perform distinct sets of effector functions. B cells diversify and rearrange their Ig constant regions through class-switch recombination, a process by which the constant region is switched from one Ig isotype to another, namely from IgM and IgD to IgG, IgA and IgE (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region of Ig gamma-1 (IgG1) isotype interacts (via the fragment crystallizable, Fc) with receptors on innate immune cells and the complement system to mediate humoral effector functions, including antibody-dependent cellular cytotoxicity or phagocytosis, complement-dependent cytotoxicity and inflammatory responses.

See full target information IGHG1

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文献 (37)

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websiteProtocolBooklet

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Human IgG ELISA Kit