AB196263

Human IgA ELISA Kit

Name: Human IgA ELISA Kit (ab196263) | Abcam
Brand: Abcam Limited
SKU: AB196263
Price: 118000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Recombinant
SimpleStep
詳細を見る

(1 Review)

(17 Publications)

Human IgA ELISA kit is a single-wash 90-min SimpleStep ELISA used to quantification of human Immunoglobulin A (IgA) with a sensitivity of 0.25 ng/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Broad sample compatibility – works with saliva, urine, plasma, cell culture supernatants, milk, and serum
- Cited in over 10 publications

別名を表示する

Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, IGHA1

7 Images

ELISA

Supplier Data

ELISA - Human IgA ELISA Kit (AB196263)

sELISA

Supplier Data

Sandwich ELISA - Human IgA ELISA Kit (AB196263)

Comparison of IgA levels in Human serum, milk, saliva, and urine.

Fluids from a pooled selection of healthy donors were evaluated for the presence of Human IgA in serum, milk, saliva, and urine using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution (1 : 2X10⁵). The mean levels of Human IgA in serum were found at 2.45 mg/mL, in milk at 0.74 mg/mL, in saliva at 0.94 mg/mL and in urine at 0.05 mg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IgA ELISA Kit (AB196263)

Example of Human IgA standard curve in Sample Diluent NS. The Human IgA standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IgA ELISA Kit (AB196263)

IgA levels in individual healthy donors.

Ten individual healthy donors were evaluated for the presence of IgA in serum using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution (1 : 2X10⁵). The mean level of Human IgA was found at 3.978 mg/mL with a range of 1.016 – 9.476 mg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IgA ELISA Kit (AB196263)

Linearity of dilution.

Purified IgA was spiked in culture media and diluted in a 2-fold dilution series in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human IgA ELISA Kit (AB196263)

Example of Human IgA standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IgA ELISA Kit (AB196263)

Native IgA was measured in serum, plasma citrate, plasma EDTA, plasma heparin, milk, saliva and urine in a 2-fold dilution series. Sample dilutions were made in Sample Diluent NS. Purified IgA was spiked in culture media and diluted in a 2-fold dilution series in Sample Diluent NS.

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Key facts

検出方法

Colorimetric

サンプルタイプ

Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 0.25 ng/mL

検出範囲

0.78 - 50 ng/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human IgA ELISA Kit ab196263 is a rapid single-wash 90-min ELISA kit to measure Human IgA in saliva, urine, plasma, cell culture supernatant, milk, serum. This SimpleStep ELISA® sensitivity is 0.25 ng/mL.

How the assay works
Human IgA SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit recognizes both native and purified Human IgA protein in serum, plasma, milk, urine, saliva, and cell culture media samples only. Cell and tissue extracts have not been tested with this kit.

Human IgA ELISA Kit ab196263 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human IgA ELISA Kit ab196263
Human IgA ELISA Kit ab196263 has been used to study inflammatory responses to acute pancreatitis⁽¹⁾and to gastric bypass surgery in obese patients⁽²⁾.

References:
⁽¹⁾Yuhan Wang et al. 2022, PMID: 35273695,
⁽²⁾Xiaojiang Dai et al. 2017, PMID: 28222218.

Save your precious samples
Our 384-well Human IgA ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human IgA Antibody Pair - BSA and Azide free ab242035

Related and recommended products
Human IgA ELISA Kit ab196263 is commonly used to measure immune system functioning.

Other ELISA kits to measure Immunoglobulins include:
- Human IgG ELISA kit ab195215
- Human IgM ELISA kit ab214568
- Human IgD ELISA kit ab320794
- Human IgE ELISA kit ab195216

Human Immunoglobulin A (IgA) belongs to the immunoglobulin family of proteins which include Human IgG, IgE, and IgM, and are known as antibodies. IgA has an essential role in mucosal immunity. IgA is the second most common serum Ig and is about one-fifth of the concentration of IgG, however it is the most abundant immunoglobulin in secretions, such as saliva, mucus, sweat, and tears. In normal human serum, IgA is found mainly as a monomeric, alternatively, in secretions, IgA is found as a dimer containing two additional proteins, secretory piece, and a J chain. The secretory piece is synthesized in the epithelial cells and is added to IgA as it passes into the secretions, protecting it from degradation. IgA works is with lysozymes to hydrolyze carbohydrates in bacterial cells walls to clear the infection. The most common disease associated with high levels of IgA in the kidney is IgA nephropathy, aka Berger's disease. There is no clear clarification as to why IgA deposits occur in the kidney, however abnormality in the immune system is a possibility. Other diseases that IgA is associated with are Celiac disease and Henoch-Schonlein purpura (HSP).

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.92" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.12" } ]

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回収率

[ { "sample": "Milk", "range": "81 - 101.51 %", "average": "= 92.21" }, { "sample": "Serum", "range": "81.35 - 109.17 %", "average": "= 98.37" }, { "sample": "Urine", "range": "86.21 - 109.34 %", "average": "= 99.48" }, { "sample": "Saliva", "range": "87.91 - 92.45 %", "average": "= 90.31" }, { "sample": "Cell culture media", "range": "85.17 - 117.29 %", "average": "= 100.65" }, { "sample": "Citrate plasma", "range": "88.78 - 98.99 %", "average": "= 93.47" }, { "sample": "EDTA Plasma", "range": "75.01 - 87.76 %", "average": "= 81.23" }, { "sample": "Heparin Plasma", "range": "91.05 - 109.18 %", "average": "= 97.17" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Immunoglobulin A (IgA) also known as sIgA in its secretory form is an important component of the immune system. With a molecular weight of approximately 160 kDa IgA exists as a monomeric or dimeric structure. It is primarily expressed in mucosal areas such as the respiratory tract gastrointestinal tract and urogenital tract while also being present in secretions like saliva tears and breast milk. In its dimeric form IgA associates with a joining (J) chain and a secretory component which facilitates its transport across mucosal barriers.

Biological function summary

IgA functions as a first line of defense in immune responses occurring at mucosal surfaces. Its primary role is to neutralize pathogens toxins and prevent their attachment and penetration through epithelial cells. This antibody does not form part of a larger complex but plays an important role in immune exclusion by trapping antigens in the mucus layer. Additionally IgA can mediate antibody-dependent cellular cytotoxicity and engage with specific receptors like FcαRI (CD89) on immune cells further enhancing its protective capabilities.

Pathways

IgA participates in the mucosal immune response which is a sub-component of the broader immune system pathways. It is closely associated with the polymeric immunoglobulin receptor (pIgR) which transports IgA to the mucosal surfaces. The pathway involves recombination activation proteins (RAG) during its class-switching process in B cells differentiating from IgM-producing cells to those secreting IgA. These pathways are integral to maintaining the mucosal environment and protecting against pathogens.

Alterations or deficiencies in IgA can lead to increased susceptibility to infections and disorders like IgA nephropathy and celiac disease. In IgA nephropathy IgA deposits in the kidneys result in inflammation and damage to renal tissues. Celiac disease involves an immune reaction to gluten where IgA antibodies against tissue transglutaminase (tTG) contribute to intestinal damage. Both conditions illustrate the importance of IgA in maintaining immune balance and its connection with disease pathologies.

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製品プロトコール

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ターゲットの情報

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed : 2241915).

See full target information IGHA1

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文献 (17)

Recent publications for all applications. Explore the full list and refine your search

Pediatric gastroenterology, hepatology & nutrition 27:322-331 PubMed39319280

2024

Relationship of MicroRNA according to Immune Components of Breast Milk in Korean Lactating Mothers.

Applications

Unspecified application

Species

Unspecified reactive species

You Jin Choi,Da Hye Lee,Jeonglyn Song,Ki-Uk Kim,Hyeyoung Min,Sung-Hoon Chung,Tae Hyeong Kim,Chae-Young Kim,Insoo Kang,Na Mi Lee,Dae Yong Yi

Frontiers in nutrition 10:1254712 PubMed38455871

2024

The differential effect of two cereal foods on gut environment: a randomized, controlled, double-blind, parallel-group study.

Applications

Unspecified application

Species

Unspecified reactive species

Yohsuke Yamauchi,Hirofumi Masutomi,Katsuyuki Ishihara,Tenagy Hartanto,Chol Gyu Lee,Shinji Fukuda

The Journal of clinical investigation 134: PubMed38015619

2023

Disease-associated AIOLOS variants lead to immune deficiency/dysregulation by haploinsufficiency and redefine AIOLOS functional domains.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Sun Kuehn,Inga S Sakovich,Julie E Niemela,Agustin A Gil Silva,Jennifer L Stoddard,Ekaterina A Polyakova,Ana Esteve Sole,Svetlana N Aleshkevich,Tatjana A Uglova,Mikhail V Belevtsev,Vladislav R Vertelko,Tatsiana V Shman,Aleksandra N Kupchinskaya,Jolan E Walter,Thomas A Fleisher,Luigi D Notarangelo,Xiao P Peng,Ottavia M Delmonte,Svetlana O Sharapova,Sergio D Rosenzweig

Nature communications 14:6959 PubMed37907518

2023

Peripheral helper-T-cell-derived CXCL13 is a crucial pathogenic factor in idiopathic multicentric Castleman disease.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Harada,Yoshikane Kikushige,Toshihiro Miyamoto,Kazuko Uno,Hiroaki Niiro,Atsushi Kawakami,Tomohiro Koga,Koichi Akashi,Kazuyuki Yoshizaki

Frontiers in nutrition 9:1078060 PubMed36698463

2023

Dietary supplement of mushrooms promotes SCFA production and moderately associates with IgA production: A pilot clinical study.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichiro Nishimoto,Junya Kawai,Koichiro Mori,Tenagy Hartanto,Kaori Komatsu,Toru Kudo,Shinji Fukuda

Journal of clinical medicine 11: PubMed35956128

2022

Lower Levels of ABO Anti-A and Anti-B of IgM, IgG and IgA Isotypes in the Serum but Not the Saliva of COVID-19 Convalescents.

Applications

Unspecified application

Species

Unspecified reactive species

Eva M Matzhold,Günther F Körmöczi,Chiara Banfi,Marlies Schönbacher,Camilla Drexler-Helmberg,Ivo Steinmetz,Andrea Berghold,Peter Schlenke,Gabriel E Wagner,Anja Stoisser,Barbara Kleinhappl,Wolfgang R Mayr,Thomas Wagner

EMBO molecular medicine 14:e15326 PubMed35393790

2022

BNT162b2 vaccine induces antibody release in saliva: a possible role for mucosal viral protection?

Applications

Unspecified application

Species

Unspecified reactive species

Abbass Darwich,Chiara Pozzi,Giulia Fornasa,Michela Lizier,Elena Azzolini,Ilaria Spadoni,Francesco Carli,Antonio Voza,Antonio Desai,Carlo Ferrero,Luca Germagnoli,Alberto Mantovani,Maria Rescigno

American journal of translational research 14:918-926 PubMed35273695

2022

Ulinastatin combined with glutamine improves liver function and inflammatory response in patients with severe acute pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Zhao,Yanrong Ma,Qiuyue Li,Yuhan Wang

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 53:903-920 PubMed35138631

2022

Characterization of two new strains of Lactococcus lactis for their probiotic efficacy over commercial synbiotics consortia.

Applications

Unspecified application

Species

Unspecified reactive species

Biplab Bandyopadhyay,Satinath Das,Prashanta Kumar Mitra,Ashutosh Kundu,Vivekananda Mandal,Rajsekhar Adhikary,Vivekananda Mandal,Narayan Chandra Mandal

International journal of molecular sciences 23: PubMed35008744

2021

Evaluation of the Relationships between Intestinal Regional Lymph Nodes and Immune Responses in Viral Infections in Children.

Applications

Unspecified application

Species

Unspecified reactive species

Yayoi Aoki,Tomoya Ikeda,Naoto Tani,Miho Watanabe,Takaki Ishikawa

View all publications

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Human IgA ELISA Kit