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Human IFN gamma High Sensitivity ELISA Kit (ab46048)

Submit a review Q&A (7)References (3)
ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)
  • ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)
  • ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)
  • ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)
  • Typical Standard Curve

Key features and details

  • Sensitivity: 0.69 pg/ml
  • Range: 0.78 pg/ml - 25 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human IFN gamma High Sensitivity ELISA Kit
    Interferon gamma キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Overall 3.9%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Overall 8.6%

  • サンプルの種類

    Cell culture supernatant, Serum, Plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    < 0.69 pg/ml

  • 検出範囲

    0.78 pg/ml - 25 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 108 % - %
    Serum 92 % - %
    Plasma 72 % - %

  • 全工程の試験時間

    3h 00m

  • ステップ

    Multiple steps standard assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Abcam’s IFN gamma Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IFN gamma in Human sera, plasmas, buffered solutions and cell culture media.


    A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples. 


    This kit will recognize both endogenous and recombinant Human IFN gamma.


    Get results in 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range. 

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 2 x 96 tests 1 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml
    200X Wash Buffer White 2 x 10ml 1 x 10ml
    Amplification Diluent Brown & blue spot 1 x 25ml 1 x 15ml
    Amplifier Yellow 2 x 200µl 1 x 200µl
    Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml
    Biotinylated anti-IFN gamma Red 2 x 0.4ml 1 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml
    HRP Diluent Red 2 x 23ml 1 x 23ml
    IFN gamma Microplate (12 x 8 well strips) 2 units 1 unit
    IFN gamma Standard (Lyophilized) Yellow 4 vials 2 vials
    Plastic Plate Covers 4 units 2 units
    Stop Reagent Black 2 x 11ml 1 x 11ml
    Streptavidin-HRP 4 x 5µl 2 x 5µl

  • 研究分野

  • 機能

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

  • 組織特異性

    Released primarily from activated T lymphocytes.

  • 関連疾患

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

  • 配列類似性

    Belongs to the type II (or gamma) interferon family.

  • 翻訳後修飾

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 別名

    • IF 1
    • IFG
    • IFI
    • ifn g
    • IFN gamma
    • IFN immune
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    • Type II Interferon
    see all

  • 参照データベース

    関連製品

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab46048の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.
    特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.

    画像

    • ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)
      ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)

      IFN gamma measured in cell culture supernatants from control or treated (48 hours; 2% PHA-M; LifeTechnologies) PBMCs, diluted 1/100-1/10000 (duplicates +/- SD).

    • ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)
      ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)

      IFN gamma measured in undiluted cell culture supernatants from control or stimulated (48 hours with 50 ng x mL-1 PMA (ab120297) and 1 μM ionomycin (ab120116)) Jurkat cells. Control sample was below limit of detection (<0.57 pg x mL-1)  (duplicates +/- SD).

    • ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)
      ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)

      IFN gamma measured in undiluted human biological fluids; mouse and rat samples (plasma and serum) were below limit of detection (duplicates +/- SD).

    • ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)
      ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)

      Dilution curves of IFN gamma (ab46048) and NIBSC standard (87/586). One ng of standard IFN gamma corresponds to 12.3 (+/- 1.3) IU NIBSC 87/586. Background signal subtracted (duplicates; +/- SD).

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab46048

    参考文献 (3)

    ab46048 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab46048 は 3 報の論文で使用されています。

    • Xu W  et al. Alterations in bile acids as metabolic signatures in the patients with human adenovirus type 7 infection. Front Med (Lausanne) 9:896409 (2022). PubMed: 36160124
    • Wang X  et al. Penehyclidine hydrochloride alleviates lipopolysaccharide-induced acute respiratory distress syndrome in cells via regulating autophagy-related pathway. Mol Med Rep 23:N/A (2021). PubMed: 33300058
    • Weng J  et al. CD44 3'-Untranslated Region Functions as a Competing Endogenous RNA to Enhance NK Sensitivity of Liver Cancer Stem Cell by Regulating ULBP2 Expression. Int J Biol Sci 15:1664-1675 (2019). PubMed: 31360109

    レビューと Q&A

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    1-7 of 7 Abreviews or Q&A

    Question

    looking for ELISA kit for IL2, IL4, IL10 and IFN that works both with human and mouse
    are any of these kits crossreactive with human or mouse?

    Read More
    Answer

    ab46048, human High Sensitivity IFNg ELISA kit, does not cross react with mouse sample.

    Read More

    Question

    Hi,

    Can you please let me know what the expiration date for kit ab46048 is?

    Thank you!

    Read More
    Answer

    Thank you very much for contacting us with your question.

    We don't assign specific expiration dates for our products, but we expect the kit ab46048 to be viable for 12-18 months. I've looked at the specific lots on our most recent orders to your institution, and we would have removed these kits from our stock in June/July of next year. Our Abpromise guarantees that all products will work as stated on the datasheets for up to 6 months after purchase.

    I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

    Read More

    Question

    Inquiry: Hi, 1) For the kit ab46048, can the reconstituted standard at 25 pg/mL be stored at 2-8C until the expiration of the kit and be reused? 2) The Wash buffer which is included with the kit is this PBS based? Can PBST be substituted as the wash beffer? Thank you,

    Read More
    Answer

    Thank you for contacting us with your questions, and for your patience while I have been in touch with the lab regarding your enquiries.
    The lab scientists do not recommend storing the reconstituted standard because it is not stable and it may not work after storage. The wash buffer can be substitued with PBS with 0.05% Tween.

    I hope this information will be useful, but please let me know if you have further questions or if there is anything else that we can do for you, and I'll be happy to help.

    Read More

    Question

    1. Is the standard in this kit available as a stand-alone item?
    2. The protocol states that the reconstituted standards cannot be stored. We need to do a quality control assay of the standard before running the assay the next day. Can the 25pg/ml stock solution be stored? If so, what is the recommended temperature?

    Read More
    Answer

    Thank you for contacting us.

    I have confirmed that the standard for the Interferon Gamma Human ELISA Kit, ab46048, is not available as a stand-alone item.

    The laboratory that developed the assay warns against storing the standard:

    "We do not recommend to store any reconstituted standards, even at -20°C; our tests shows that the molecule is not stable at this concentration."


    Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Our customer is looking for  IFN gamma ELISA kit for bovine and guinea pig.   His samples are serum and plasma.   I have checked there's no product for bovine and guinea pig sample but,   please check the product which has possibility to cross react.

    Read More
    Answer

    Thank you for your patience. I have checked on the bovine IFN-gamma ELISA Kit. Unfortunately, the kit currently is not available in our catalog as the quality control tests have not yet been completed.  In general, due to the low homology between the human and bovine as well as guinea pig proteins, a human IFN gamma ELISA kit might most likely not work well for these two species, very unfortunately. I also checked our IFN gamma antibodies that were tested in ELISA, but unfortunately, we do not have any that were tested in bovine or guinea pig either. I am sorry to have no better news for you at this moment. Please do not hesitate to contact us if you need any more advice or information. 

    Read More

    Question

    Can this TNF-a Elisa kit be used to detect TNFa in human  Plasma samples? What is the cost per kit and could we get a special evaluation price? Do you also sell sensitive kits for IFN-g? Kind regards  

    Read More
    Answer

    Thank you for contacting us. I can confirm that ab46087 can be used to detect TNF alpha in human plasma samples. The cost per KIT can be obtained from our Australian distributor Sapphire Bioscience. Their address is as follows. Please discuss with them for special offers; Sapphire Bioscience Pty Ltd 126 Cope Street Waterloo - NSW 2017 Australia Tel +61296982022, 1800062088 Website http://www.sapphirebioscience.com Fax +61296981022 Email sales@sapphirebioscience.com We have ab46025 and ab46048 to detect human IFNg in human plasma. Please check the datasheets for detection limits of these. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

    Read More

    Question

    Hi, We have recently purchased and run a couple of High sensitivity IFN-y ELISA kits (code ab46048). Whilst the assay worked okay and the standard curve was fine, we were a bit concerned that the OD for the top standard was only 0.4 OD units, I have run this assay before and even then the top standard OD was only ~0.7 -0.8OD units. I see in the kit insert, the example figure suggests that a top OD of ~ 2 units should be expected. Can you shed any light on why this may have occurred? We have performed the assay exactly as specified in the insert without any modifications, the only thing I can think of is the temperature of the laboratory - the insert recommends 18-25 C, though our laboratory is at the low end (~ 17- 20 C), could this relatively small change in temperature account for the large discrepancy in OD? As an aside, on both occasions I've run this assay, we've also noticed that we haven't been provided with enough HRP diluent in the kit, it says that it should provide you with one 25ml bottle, but in actual fact only ~ 23 ml was provided. Thanks,

    Read More
    Answer

    Thanks for your enquiry. I am very sorry to hear that you are having problems with this product. I have been discussing this enquiry with my colleagues in the Lab and would like to share the followings. 1) First, the regular volume of HRP diluent normally provided is 25.0ml and I will internally investigate how vials of only 23.0ml could have been dispatched. We can provide additional vial FOC for you if you wish. 2) Regarding low OD observed, the temperature can indeed be an explanation. The effect of lower temperature has not been fully studied and I have no data to show. However, generally based on physical-chemistry principles the temperature impacts the enzyme reactivity. In the High sensitivity kits configuration, enzyme is incubated twice (2 steps + the amplifier step) so it makes sense to consider high impact. 3) The main important factor for the good validation of the assay is the ratio between 0 and 25pg/ml standard point with 0.8 OD and less than 0.08 for zero, the assay remains fine. Hope this helps. Please do let me know how you wish to proceed with this enquiry.

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