Human IFN gamma ELISA Kit (ab46025)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 12.5 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IFN gamma ELISA Kit
Interferon gamma キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Sample 30 4.9% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Sample 30 10% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 5 pg/ml -
検出範囲
12.5 pg/ml - 400 pg/ml -
添加回収試験
107 %
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全工程の試験時間
2h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human interferon gamma ELISA kit is designed for the quantitative measurement of IFN gamma in supernatants, buffered solutions or serum and plasma samples and other body fluids.
A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.
This kit will recognize both endogenous and recombinant Human IFN gamma.
Get higher sensitivity in only 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 2 x 96 tests 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml 1 x 7.5ml 1 x 13ml Biotinylated anti-IFN gamma Red 1 x 400µl 2 x 400µl 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml 1 x 11ml 1 x 24ml Control Silver 2 vials 4 vials 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml 1 x 12ml 1 x 23ml IFN gamma Microplate (12 x 8 well strips) 1 unit 2 units 1 unit 2 units IFN gamma Standard (Lyophilized) Yellow 2 vials 4 vials 2 vials 4 vials Standard Diluent (Serum) 1 x 7ml 2 x 7ml 1 x 7ml 2 x 7ml Stop Reagent Black 1 x 11ml 2 x 11ml 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl 2 x 5µl 4 x 5µl -
研究分野
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機能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
組織特異性
Released primarily from activated T lymphocytes. -
関連疾患
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
配列類似性
Belongs to the type II (or gamma) interferon family. -
翻訳後修飾
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
細胞内局在
Secreted. - Information by UniProt
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別名
- IF 1
- IFG
- IFI
see all -
参照データベース
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
関連製品
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Related Products
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SimpleStep ELISA kits
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46025の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Sandwich ELISA - IFN gamma Human ELISA Kit (ab46025)
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Typical Standard CurveRepresentative Standard Curve using ab46025
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab46025 は 15 報の論文で使用されています。
- Ye T et al. LncRNA CCAT1 is overexpressed in tuberculosis patients and predicts their survival. Immun Inflamm Dis 10:218-224 (2022). PubMed: 34847295
- Long C et al. B7-H3 as a Target for CAR-T Cell Therapy in Skull Base Chordoma. Front Oncol 11:659662 (2021). PubMed: 34868903
- Yang L et al. Long non-coding RNA GAS5 protects against Mycoplasma pneumoniae pneumonia by regulating the microRNA-222-3p/TIMP3 axis. Mol Med Rep 23:N/A (2021). PubMed: 33760178
- Ye Y et al. miR-489-3p inhibits TLR4/NF-?B signaling to prevent inflammation in psoriasis. Exp Ther Med 22:744 (2021). PubMed: 34055060
- Zhao J et al. Optimization of GPC3-specific chimeric antigen receptor structure and its effect on killing hepatocellular carcinoma cells. Bioengineered 12:3674-3683 (2021). PubMed: 34261411