Human ICAM3 ELISA Kit (CD50) (ab46024)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.62 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human ICAM3 ELISA Kit (CD50) -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Sample A 8 19.75ng/ml 0.172 0.86% Sample B 8 2.83ng/ml 0.099 3.49% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Sample A 36 19.51ng/ml 0.391 1.99% Sample B 36 2.71ng/ml 0.176 6.27% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 0.3 ng/ml -
検出範囲
0.62 ng/ml - 20 ng/ml -
全工程の試験時間
1h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s ICAM3 (CD50) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ICAM3 in serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific for ICAM3 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known ICAM3 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for ICAM3 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of ICAM3 present in the samples.
This kit will recognize both endogenous and recombinant Human ICAM3.
-
アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-ICAM-3 (CD50) Red 1 x 0.4ml 2 x 0.4ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 12ml 1 x 23ml ICAM3 Control Silver 2 vials 4 vials ICAM3 Microplate (12 x 8 well strips) 1 unit 2 units ICAM3 Standard Yellow 2 vials 4 vials Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究分野
-
関連性
ICAM3 is a member of the intercellular adhesion molecule family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM3 is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. -
細胞内局在
Cell Membrane; single-pass type I membrane protein -
別名
- CD50
- CDW50
- ICAM 3
see all -
参照データベース
- Entrez Gene: 3385 Human
- SwissProt: P32942 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46024の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
-
Typical Standard Curve
Representative Standard Curve using ab46024
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab46024 は論文での使用が確認できていません。