Human ICAM1 ELISA Set (without plates) (ab47349)
製品の概要
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製品名
Human ICAM1 ELISA Set (without plates)
ICAM1 キット 製品一覧 -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 0.1 ng/ml -
検出範囲
0.25 ng/ml - 8 ng/ml -
全工程の試験時間
1h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s ICAM1 Human in vitro ELISA Set is designed for the quantitative measurement of ICAM1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA set is intended to measure human ICAM1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
The ICAM1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human ICAM1.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 10 x 96 tests 5 x 96 tests 15 x 96 tests Chromogen TMB Substrate Solution 4 vials 2 vials 6 vials Detection biotinylated anti-ICAM1 antibody 2 x 0.55ml 1 x 0.55ml 3 x 0.55ml ICAM1 Capture Antibody 2 x 0.5ml 1 x 0.5ml 3 x 0.5ml ICAM1 Standard 10 vials 5 vials 15 vials Streptavidin-HRP 2 vials 1 vial 3 vials -
研究分野
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機能
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
配列類似性
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
翻訳後修飾
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
細胞内局在
Membrane. - Information by UniProt
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別名
- Antigen identified by monoclonal antibody BB2
- BB 2
- BB2
see all -
参照データベース
- Entrez Gene: 3383 Human
- Omim: 147840 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47349の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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特記事項 |
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Sandwich ELISA
Use at an assay dependent concentration. |
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab47349 は 3 報の論文で使用されています。
- Meneses GC et al. Endothelial biomarkers as predictors for haemodialysis need in severe leptospirosis patients (Weil's disease). Trop Med Int Health 27:727-734 (2022). PubMed: 35761507
- Tessarolo LD et al. Endothelial activation is associated with albuminuria in multibacillary leprosy. Rev Inst Med Trop Sao Paulo 63:e44 (2021). PubMed: 34161550
- Lino DOC et al. Interleukin-6 and adhesion molecules VCAM-1 and ICAM-1 as biomarkers of post-acute myocardial infarction heart failure. Braz J Med Biol Res 52:e8658 (2019). PubMed: 31778438