Human Anti-Herpes simplex virus Type 1 and 2 IgG ELISA Kit (HSV1+2) (ab108741)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
製品の概要
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製品名
Human Anti-Herpes simplex virus Type 1 and 2 IgG ELISA Kit (HSV1+2) -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Pos.Serum 13 5.8% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Pos.Serum 3 3.6% -
サンプルの種類
Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Indirect -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s anti-Herpes simplex virus Type 1 and 2 (HSV1+2) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Herpes simplex virus Type 1 and 2 in Human serum and plasma.
A 96-well plate has been precoated with Herpes simplex virus Type 1 and 2 antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Herpes simplex virus Type 1 and 2-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Herpes simplex virus Type 1 and 2 IgG sample captured in plate.
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アプリケーション
適用あり: ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit Herpes simplex virus Type 1+2 (IgG) Coated Microplate (12 x 8 wells) 1 unit Herpes simplex virus Type 1+2 anti-IgG HRP Conjugate Blue, Black cap 1 x 20ml Herpes simplex virus Type 1+2 IgG Cut-off Control Yellow, Green cap 1 x 3ml Herpes simplex virus Type 1+2 IgG Negative Control Yellow, Blue cap 1 x 2ml Herpes simplex virus Type 1+2 IgG Positive Control Yellow, Red cap 1 x 2ml IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究分野
関連製品
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108741の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
画像
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab108741 は論文での使用が確認できていません。