AB108861

Human Heat Shock Protein 47 ELISA Kit (SERPINH1)

Name: Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab108861) | Abcam
Brand: Abcam Limited
SKU: AB108861
Price: 109000 JPY
Availability: InStock

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(2 Publications)

Human Heat Shock Protein 47 ELISA Kit (SERPINH1) is a Sandwich (quantitative) ELISA for the measurement of Human Heat Shock Protein 47 (SERPINH1) in Human in Biofluids, Cell/Tissue Extracts samples.

別名を表示する

CBP1, CBP2, HSP47, SERPINH2, PIG14, SERPINH1, Serpin H1, 47 kDa heat shock protein, Arsenic-transactivated protein 3, Cell proliferation-inducing gene 14 protein, Collagen-binding protein, Rheumatoid arthritis-related antigen RA-A47, AsTP3, Colligin

1 Images

Sandwich ELISA - Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (AB108861)

sELISA

Supplier Data

Sandwich ELISA - Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (AB108861)

Representative Standard Curve using ab108861.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cerebral Spinal Fluid, Plasma, Serum, Tissue, Cell Lysate

交差種

Human

アッセイタイプ

Sandwich (quantitative)

検出感度

= 0.54 ng/mL

検出範囲

1.563 - 100 ng/mL

アッセイ時間

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Abcam's Heat Shock Protein 47 (SERPINH1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP47 levels in Human plasma, milk, serum, cell lysate, cerebrospinal fluid and tissue samples.

A HSP47 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP47 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP47 captured in plate.

Get results in 90 minutes with Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab202407) from our SimpleStep ELISA^® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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精度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

Multi

保管に関する情報

Please refer to protocols

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Hsp47 also known as the heat shock protein 47 is a molecular chaperone with a molecular weight of approximately 47 kDa. It mainly resides in the endoplasmic reticulum and participates in collagen synthesis. Hsp47 binds specifically to unfolded procollagen chains ensures their proper folding and prevents premature aggregation. Due to its specialized function Hsp47 is highly expressed in collagen-producing cells like fibroblasts. Researchers commonly study this protein using techniques like Hsp47 ELISA to measure its expression or activity levels.

Biological function summary

Hsp47 ensures the quality control of collagen within the secretory pathway. It does not form part of any large protein complexes but operates closely with collagen molecules. By stabilizing procollagen's triple-helix structure Hsp47 plays an important role in collagen biosynthesis. Dysfunctional collagen processes can arise without this protein's presence potentially leading to cellular stress or structural weaknesses within tissues rich in collagen.

Pathways

Hsp47's activity integrates into the collagen synthesis and secretion pathways. This protein is related to other molecular chaperones such as Hsp70 that assist in maintaining protein homeostasis within the cell. Hsp47’s function links to fibrosis pathways because of its role in collagen maturation. In terms of fibrotic pathologies this involvement makes the chaperoning of procollagen a critical control point.

Disrupted Hsp47 function frequently associates with fibrotic conditions and osteogenesis imperfecta. Fibrosis results from excessive collagen deposition in which Hsp47 is deeply implicated. Additionally certain cancer types have shown altered Hsp47 expression correlating with increased cancer cell invasiveness due to extracellular matrix remodeling. Studies have noted interactions between Hsp47 and TGF-beta signaling in fibrotic tissues reinforcing its role in pathological collagen turnover.

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製品プロトコール

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ターゲットの情報

Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.

See full target information SERPINH1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Proteomics 16:2718-2731 PubMed27459910

2016

Skin aging caused by intrinsic or extrinsic processes characterized with functional proteomics.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-You Fang,Pei-Wen Wang,Chun-Hsun Huang,Mu-Hong Chen,Yun-Ru Wu,Tai-Long Pan

Protein & cell 6:589-598 PubMed26091621

2015

Involvement of collagen-binding heat shock protein 47 in scleroderma-associated fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Chu,Ting Wu,Wenyu Wu,Wenzhen Tu,Shuai Jiang,Sidi Chen,Yanyun Ma,Qingmei Liu,Xiaodong Zhou,Li Jin,Jiucun Wang

View all publications

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Human Heat Shock Protein 47 ELISA Kit (SERPINH1)