Human Haptoglobin ELISA Kit (ab108858)
Key features and details
- Sensitivity: 1.1 ng/ml
- Range: 3.125 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Saliva, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Haptoglobin ELISA Kit
Haptoglobin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5.8% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 10.8% -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
1.1 ng/ml -
検出範囲
3.125 ng/ml - 200 ng/ml -
添加回収試験
= 97 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Haptoglobin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Haptoglobin concentrations in Human urine, saliva, milk and cell culture supernatants.
A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Haptoglobin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Haptoglobin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Haptoglobin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Haptoglobin Microplate (12 x 8 well strips) 1 unit Haptoglobin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Anhaptoglobinemia -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Binding peptide
- BP
- Haptoglobin alpha chain
see all -
参照データベース
- Entrez Gene: 3240 Human
- Omim: 140100 Human
- SwissProt: P00738 Human
- Unigene: 513711 Human
- Unigene: 702099 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108858の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab108858.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab108858 は 3 報の論文で使用されています。
- Huang M et al. Identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis. Front Neurol 13:886040 (2022). PubMed: 36003300
- Wagener BM et al. Role of heme in lung bacterial infection after trauma hemorrhage and stored red blood cell transfusion: A preclinical experimental study. PLoS Med 15:e1002522 (2018). PubMed: 29522519
- Oh JY et al. Absorbance and redox based approaches for measuring free heme and free hemoglobin in biological matrices. Redox Biol 9:167-177 (2016). PubMed: 27566280