AB235635

Human Granzyme B ELISA Kit

Recombinant
SimpleStep
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Human Granzyme B ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Granzyme B with a sensitivity of 106 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

別名を表示する

CGL1, CSPB, CTLA1, GRB, GZMB, Granzyme B, C11, CTLA-1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, Granzyme-2, Human lymphocyte protein, SECT, T-cell serine protease 1-3E, CTSGL1, Lymphocyte protease, HLP

4 Images

ELISA - Human Granzyme B ELISA Kit (AB235635)

ELISA

Supplier Data

ELISA - Human Granzyme B ELISA Kit (AB235635)

sELISA

Supplier Data

Sandwich ELISA - Human Granzyme B ELISA Kit (AB235635)

Interpolated concentrations of native Granzyme B in human PBMC cell culture supernatant samples treated for 46 hours with 1.5% PHA-M.

The concentrations of Granzyme B were measured in duplicates, interpolated from the Granzyme B standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme B concentration was determined to be 44.1 ng/ml in treated PBMC supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human Granzyme B ELISA Kit (AB235635)

Example of human Granzyme B standard curve in Sample Diluent NS + Enhancer.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Granzyme B ELISA Kit (AB235635)

Representative image of Human Granzyme B ELISA Kit (ab235635)

Components shown from left to right :

- Sample diluent NS

- 10X Human Granzyme B Capture Antibody

- 10X Human Granzyme B Detector Antibody

- Human Granzyme B Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent CPI2

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

検出方法

Colorimetric

サンプルタイプ

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 106 pg/mL

検出範囲

156 - 10000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human Granzyme B ELISA Kit ab235635 is a rapid single-wash 90-min Sandwich ELISA to measure Human Granzyme B in cell culture supernatant, citrate plasma, heparin plasma, serum. This SimpleStep sensitivity is 106 pg/mL.

How the assay works

Human Granzyme B SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Granzyme B ELISA Kit ab235635 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Granzyme B antibody [EPR20129-233] - BSA and Azide free (Capture) ab244767
- Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (Detector) ab245038

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精度

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.5" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "100 - 124 %", "average": "= 110" }, { "sample": "Serum", "range": "87 - 94 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "90 - 99 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "94 - 99 %", "average": "= 97" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme B also known as GZMB GB11 or granzyme B protein is a serine protease with a molecular mass of approximately 32 kDa. It is expressed mainly in cytotoxic T lymphocytes and natural killer (NK) cells. This enzyme plays a mechanical role in inducing apoptosis in target cells serving as an effector protein in the immune system's defense against virally infected cells or transformed cancer cells. The activity of granzyme B relies on its ability to cleave after aspartate residues in substrate proteins leading to the activation of apoptotic pathways.

Biological function summary

Granzyme B participates prominently in the immune response by activating caspases particularly caspase-3 which promotes the breakdown of cellular components necessary for apoptosis. Granzyme B does not function in isolation but acts in concert with other immune system factors such as perforin to effectively induce cell death. Perforin creates pores in the target cell membrane allowing granzyme B to enter and instigate the apoptosis sequence. The enzyme also contributes to the processing of cytokines which enhances the immune response further.

Pathways

Studies have determined that granzyme B is critical in the apoptosis pathway particularly in the granule exocytosis pathway. It closely interacts with proteins such as perforin and other granzymes to mediate apoptosis in target cells. Granzyme B also plays a role in the inflammatory response and can influence pathways associated with cytotoxic T cell signaling. Its pathway interactions ensure effective elimination of damaged or infected cells maintaining tissue homeostasis.

Granzyme B has associations with autoimmune diseases and cancer. Abnormally high levels of granzyme B can contribute to tissue damage and inflammation in autoimmune conditions like rheumatoid arthritis. In the context of cancer granzyme B aids in tumor surveillance and destruction when functioning correctly but impaired granzyme B activity can lead to evasion of immune detection by cancerous cells. Perforin also plays a role in these conditions closely working with granzyme B to either protect against or drive disease progression.

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製品プロトコール

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ターゲットの情報

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 1985927, PubMed : 3262682, PubMed : 3263427). It cleaves after Asp (PubMed : 1985927, PubMed : 8258716). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed : 31953257, PubMed : 32188940). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed : 9852092). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity).

See full target information GZMB

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1512107 PubMed40013144

2025

NALCN expression is down-regulated and associated with immune infiltration in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xuewei Li,Na Wu,Chen Wang,Beibei Pei,Xiaoyan Ma,Jun Xie,Wenhui Yang

Molecular carcinogenesis 64:162-175 PubMed39451071

2024

FOS-Mediated PLCB1 Induces Radioresistance and Weakens the Antitumor Effects of CD8 T Cells in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxian Shu,Jun Lan,Huijing Luo,Huiying Fu,Xuhuang Xiao,Liping Yang

Discover oncology 15:562 PubMed39404969

2024

Expression of CLDN1 and EGFR in PTC.

Applications

Unspecified application

Species

Unspecified reactive species

JunJie Wu,YouMei Wang,Lei Yan,YaWen Dong

Cancer immunology research 12:1691-1702 PubMed39189923

2024

CAR T Cells Engineered to Secrete IFNκ Induce Tumor Ferroptosis via an IFNAR/STAT1/ACSL4 Axis.

Applications

Unspecified application

Species

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Yaoxin Gao,Shasha Liu,Yifan Huang,Hui Wang,Yuyu Zhao,Xuyang Cui,Yajing Peng,Feng Li,Yi Zhang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2406633 PubMed39116343

2024

PTBP3 Mediates IL-18 Exon Skipping to Promote Immune Escape in Gallbladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Zhao,Jing-Wei Zhao,Yu-Han Zhang,Yi-di Zhu,Zi-Yi Yang,Shi-Lei Liu,Qiu-Yi Tang,Yue Yang,Hua-Kai Wang,Yi-Jun Shu,Ping Dong,Xiang-Song Wu,Wei Gong

eLife 12: PubMed38885133

2024

Expression of modified FcγRI enables myeloid cells to elicit robust tumor-specific cytotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Leen Farhat-Younis,Manho Na,Amichai Zarfin,Aseel Khateeb,Nadine Santana-Magal,Alon Richter,Amit Gutwillig,Diana Rasoulouniriana,Annette Gleiberman,Lir Beck,Tamar Giger,Avraham Ashkenazi,Adi Barzel,Peleg Rider,Yaron Carmi

Biological & pharmaceutical bulletin 46:1260-1268 PubMed37661405

2023

Cordycepin Enhances the Cytotoxicity of Human Natural Killer Cells against Cancerous Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nipha Chaicharoenaudomrung,Phongsakorn Kunhorm,Parinya Noisa

Frontiers in cell and developmental biology 10:928140 PubMed35990606

2022

Chimeric Antigen Receptor T Cells Targeting Cell Surface GRP78 to Eradicate Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yu,Hang Zhang,Yuncang Yuan,Jie Tang,Xinchuan Chen,Ting Liu,Xudong Zhao

View all publications

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websiteProtocolBooklet

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Human Granzyme B ELISA Kit