AB246548

Human gp130 ELISA Kit

Recombinant
SimpleStep
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Human gp130 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human gp130 with a sensitivity of 24.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

CD130, Interleukin-6 receptor subunit beta, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, CDw130, Interleukin-6 signal transducer, Membrane glycoprotein 130, Oncostatin-M receptor subunit alpha, gp130, IL6ST

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Sandwich ELISA - Human gp130 ELISA Kit (AB246548)

sELISA

Supplier Data

Sandwich ELISA - Human gp130 ELISA Kit (AB246548)

Interpolated concentrations of native gp130 in human serum and plasma samples.

The concentrations of gp130 were measured in duplicates, interpolated from the gp130 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 0.75%, plasma (citrate) 0.75%, plasma (heparin) 0.75% and PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gp130 concentration was determined to be 280 ng/mL in serum, 224 ng/mL in plasma (citrate) and 223 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human gp130 ELISA Kit (AB246548)

Human gp130 Standard Curve Comparison.

Standard Curve comparison between human gp130 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human gp130 ELISA Kit (AB246548)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gp130 concentration was determined to be 459 ng/mL with a range of 320-604 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human gp130 ELISA Kit (AB246548)

Example of human gp130 standard curve in Sample Diluent NS.

The gp130 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 24.8 pg/mL

検出範囲

62.5 - 4000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human gp130 ELISA Kit (ab246548) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of gp130 protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human gp130 with 24.8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "114 - 116 %", "average": "= 115" }, { "sample": "Serum", "range": "97 - 100 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "101 - 104 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "104 - 107 %", "average": "= 106" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD130 also known as gp130 or the IL-6 signal transducer (IL6ST) is a protein weighing around 130 kDa. This receptor is encoded by the IL6ST gene and widely expressed on the surface of cells across multiple tissues. CD130 functions mechanically as a signal transducing receptor facilitating the assembly of receptor complexes necessary for the initiation of signal transduction. It plays a major role in cytokine receptor signaling particularly interacting with interleukin-6 (IL-6) family cytokines.

Biological function summary

CD130 functions as part of the high-affinity receptor complex for certain cytokines. It partners with other receptors such as CD126 to form a complex that initiates signaling pathways after ligand binding contributing to a range of immune responses. Its role is thorough across many cellular processes including differentiation proliferation and apoptosis. In addition to immune signaling CD130 is involved in several growth factor signaling pathways.

Pathways

CD130 serves a significant role in signal transduction particularly in the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway. It works in concert with related proteins like JAK1 and STAT3 to transmit signals from extracellular cytokines to the nucleus influencing gene expression. Additionally CD130 can also be found active in the MAPK/ERK pathway which is important for cellular response to growth signals.

CD130 plays a connection especially to inflammatory conditions and certain cancers. Aberrant signaling involving CD130 has been linked to disorders like rheumatoid arthritis where it contributes to the inflammation process. Additionally it relates to multiple myeloma a cancer where abnormal CD130 interactions with proteins like IL-6 may enhance cell survival and proliferation presenting potential targets for therapeutic intervention.

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製品プロトコール

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ターゲットの情報

Signal-transducing molecule (PubMed : 2261637). The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates the intracellular JAK-MAPK and JAK-STAT3 signaling pathways (PubMed : 19915009, PubMed : 2261637, PubMed : 23294003). That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3 (PubMed : 19915009, PubMed : 23294003, PubMed : 25731159). In parallel, the IL6 signaling pathway induces the expression of two cytokine receptor signaling inhibitors, SOCS1 and SOCS3, which inhibit JAK and terminate the activity of the IL6 signaling pathway as a negative feedback loop (By similarity). Also activates the yes-associated protein 1 (YAP) and NOTCH pathways to control inflammation-induced epithelial regeneration, independently of STAT3 (By similarity). Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL27RA/WSX1 and CNTFR (PubMed : 19386761). Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism (By similarity). Has a role in embryonic development (By similarity). Essential for survival of motor and sensory neurons and for differentiation of astrocytes (By similarity). Required for expression of TRPA1 in nociceptive neurons (By similarity). Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation (By similarity). Required for normal trabecular bone mass and cortical bone composition (By similarity).. Isoform 2. Binds to the soluble IL6 : sIL6R complex (hyper-IL6), thereby blocking IL6 trans-signaling. Inhibits sIL6R-dependent acute phase response (PubMed : 11121117, PubMed : 21990364, PubMed : 30279168). Also blocks IL11 cluster signaling through IL11R (PubMed : 30279168).

See full target information IL6ST

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websiteProtocolBooklet

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Human gp130 ELISA Kit