AB288175

Human GFAP ELISA Kit

Name: Human GFAP ELISA Kit (ab288175) | Abcam
Brand: Abcam Limited
SKU: AB288175
Price: 97000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Recombinant
SimpleStep
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Human GFAP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human GFAP with a sensitivity of 0.068 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Glial fibrillary acidic protein, GFAP

3 Images

ELISA

Supplier Data

ELISA - Human GFAP ELISA Kit (AB288175)

sELISA

Supplier Data

Sandwich ELISA - Human GFAP ELISA Kit (AB288175)

Interpolated concentrations of human GFAP in human cerebrospinal fluid. Interpolated concentration of native GFAP was measured in duplicate at different sample concentrations. Undiluted samples are 100% cerebrospinal fluid. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human GFAP ELISA Kit (AB288175)

Example of human GFAP standard curve in Sample Diluent NS. Example of human GFAP standard curve. Background-subtracted data values (mean +/- SD)

Key facts

検出方法

Colorimetric

サンプルタイプ

Cerebral Spinal Fluid, Cell culture media

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 0.068 ng/mL

検出範囲

0.625 - 40 ng/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human GFAP ELISA Kit ab288175 is a rapid single-wash 90-min Sandwich ELISA to measure Human GFAP in cell culture media, CSF. This SimpleStep sensitivity is 0.068 ng/mL.

How the assay works

Human GFAP SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human GFAP ELISA Kit ab288175 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-GFAP antibody [EPR19996-132] - BSA and Azide free (Capture) ab242937
- Anti-GFAP antibody [EPR19996] - BSA and Azide free (Detector) ab242937

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精度

[ { "reproducibilityType": "Inter", "sample": "Cerebral Spinal Fluid", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.4" }, { "reproducibilityType": "Intra", "sample": "Cerebral Spinal Fluid", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.1" } ]

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回収率

[ { "sample": "Cerebral Spinal Fluid", "range": "108 - 111 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "100 - 104 %", "average": "= 102" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288175-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288175-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB288175-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288175-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GFAP Capture Antibody", "number":"AB288175-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288175-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human GFAP Lyophilized Recombinant Protein", "number":"AB288175-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288175-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288175-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288175-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GFAP Detector Antibody", "number":"AB288175-CMP08", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

See full target information GFAP

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websiteProtocolBooklet

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Abcam product promise

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Human GFAP ELISA Kit