Human GC Globulin ELISA Kit (Vitamin D Binding Protein) (ab108853)
Key features and details
- Sensitivity: 0.06 µg/ml
- Range: 0.098 µg/ml - 100 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
製品の概要
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製品名
Human GC Globulin ELISA Kit (Vitamin D Binding Protein)
Vitamin D Binding protein キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 6.2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.9% -
サンプルの種類
Serum, Plasma -
アッセイタイプ
Competitive -
検出感度
> 0.06 µg/ml -
検出範囲
0.098 µg/ml - 100 µg/ml -
添加回収試験
97 %
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全工程の試験時間
3h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human GC Globulin (Vitamin D Binding Protein) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of GC Globulin in plasma, serum, saliva, milk and cell-culture supernatants.
A GC Globulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated GC Globulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of GC Globulin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Competitive ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 2X Biotinylated Human GC Globulin (Lyophilized) 1 vial Chromogen Substrate 1 x 7ml GC Globulin Microplate (12 x 8 well strips) 1 unit GC Globulin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. -
配列類似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
細胞内局在
Secreted. - Information by UniProt
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別名
- DBP
- DBP/GC
- GC
see all -
参照データベース
- Entrez Gene: 2638 Human
- Omim: 139200 Human
- SwissProt: P02774 Human
- Unigene: 418497 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108853の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab108853.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab108853 は 6 報の論文で使用されています。
- Lasagni Vitar RM et al. Vitamin D Supplementation Impacts Systemic Biomarkers of Collagen Degradation and Copper Metabolism in Patients With Keratoconus. Transl Vis Sci Technol 11:16 (2022). PubMed: 36580321
- Vinke P et al. Prevalence of Micronutrient Deficiencies and Relationship with Clinical and Patient-Related Outcomes in Pulmonary Hypertension Types I and IV. Nutrients 13:N/A (2021). PubMed: 34836178
- Mesinovic J et al. The Relationship between Vitamin D Metabolites and Androgens in Women with Polycystic Ovary Syndrome. Nutrients 12:N/A (2020). PubMed: 32357490
- Fernando M et al. Early Pregnancy Vitamin D Binding Protein Is Independently Associated with the Development of Gestational Diabetes: A Retrospective Cohort Study. J Clin Med 9:N/A (2020). PubMed: 32664376
- Fernando M et al. Relationships between Total, Free and Bioavailable Vitamin D and Vitamin D Binding Protein in Early Pregnancy with Neonatal Outcomes: A Retrospective Cohort Study. Nutrients 12:N/A (2020). PubMed: 32824958
- Naderpoor N et al. Bioavailable and free 25-hydroxyvitamin D and vitamin D binding protein in polycystic ovary syndrome: Relationships with obesity and insulin resistance. J Steroid Biochem Mol Biol 177:209-215 (2018). Competitive ELISA . PubMed: 28734987