AB309395

Human GATA1 ELISA Kit

Name: Human GATA1 ELISA Kit (ab309395) | Abcam
Brand: Abcam Limited
SKU: AB309395
Price: 130000 JPY
Availability: InStock

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Human GATA1 ELISA Kit is a Sandwich ELISA for the measurement of Human GATA1 in Human in Cell Culture Media, Biofluids samples.

別名を表示する

ERYF1, GF1, GATA1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, NF-E1 DNA-binding protein, GATA-1, GF-1

1 Images

Sandwich ELISA - Human GATA1 ELISA Kit (AB309395)

sELISA

Supplier Data

Sandwich ELISA - Human GATA1 ELISA Kit (AB309395)

Example of standard curve using ab309395. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture supernatant, Plasma, Serum

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 16 pg/mL

検出範囲

16.38 - 4000 pg/mL

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human GATA1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GATA1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human GATA1 coated on a 96-well plate.

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回収率

[ { "sample": "Plasma", "range": "67 - 79 %", "average": "= 71.13" }, { "sample": "Serum", "range": "70 - 133 %", "average": "= 94.15" }, { "sample": "Cell culture media", "range": "86 - 106 %", "average": "= 96.31" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GATA1 also known as GATA-binding factor 1 is a transcription factor weighing approximately 42 kDa. This protein binds to the DNA sequence "GATA" within the regulatory regions of target genes to control their expression. GATA1 is mostly expressed in hematopoietic cells especially erythroid cells megakaryocytes eosinophils and mast cells. Its specific expression pattern allows it to regulate genes essential for the development and function of these cell types.

Biological function summary

GATA1 regulates gene expression and is essential for erythroid and megakaryocyte differentiation. GATA1 belongs to a family of GATA transcription factors and can form regulatory complexes with other proteins such as FOG1 (Friend of GATA1) and TAL1. These complexes facilitate the binding of GATA1 to DNA enhancing its transcriptional activity on target gene sets necessary for blood cell maturation and function.

Pathways

GATA1 participates in the erythropoiesis and megakaryopoiesis pathways. It directly regulates several genes and coordinates signals with factors like GATA2 and RUNX1 which are other key proteins involved in these pathways. Through these interactions GATA1 ensures the correct development of red blood cells and platelets maintaining hematopoietic homeostasis in the organism.

Abnormal GATA1 function is linked to blood-related disorders. Mutations or dysregulations of GATA1 can lead to diseases such as X-linked thrombocytopenia with dyserythropoietic anemia which involves low platelet count and ineffective red blood cell production. Moreover GATA1 mutations are associated with certain leukemias particularly acute megakaryoblastic leukemia. GATA1's dysfunction in these diseases often occurs in conjunction with abnormalities in related proteins like GATA2 influencing disease progression and phenotype.

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製品プロトコール

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ターゲットの情報

Transcriptional activator or repressor which serves as a general switch factor for erythroid development (PubMed : 35030251). It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed : 24245781).

See full target information GATA1

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websiteProtocolBooklet

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Human GATA1 ELISA Kit