Human Frataxin ELISA Kit (ab176112)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.1 pg/ml
- Range: 9.88 pg/ml - 800 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Frataxin ELISA Kit
Frataxin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% HeLa 9 2.3% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% HeLa 3 6.3% -
サンプルの種類
Cell culture extracts, Tissue Extracts -
アッセイタイプ
Sandwich (quantitative) -
検出感度
2.1 pg/ml -
検出範囲
9.88 pg/ml - 800 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 92 86% - 98% Cell culture media 97 93% - 101% EDTA Plasma 89 88% - 92% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Frataxin ELISA kit (ab176112) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Frataxin protein in human cell culture and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Frataxin with 1.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
ASSAY SPECIFICITY This kit recognizes both native and recombinant human Frataxin protein in cell and tissue extract samples only. Serum, plasma, saliva, and urine samples have not been tested with this kit.
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特記事項
Frataxin is a 17 kDa nuclear-encoded mitochondrial protein. In humans the gene is localized on chromosome 9 and is highly conserved during evolution. The gene is expressed in every cell, although in varying levels in different tissues and during development. The specific function of frataxin is still unknown, but it has been shown to play a role in iron metabolism. Studies have demonstrated that the deletion of the frataxin gene in yeast results in iron accumulation in mitochondria and loss of respiration. Recombinant human frataxin has been shown to bind iron in vitro, and increased mitochondrial iron levels have been observed in patients with Friedreich's Ataxia (FRDA). FRDA is an autosomal recessive, progressive degenerative disease characterized by neurodegeneration and cardiomyopathy it is the most common inherited ataxia.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 1 x 384 tests 10X Human Frataxin Capture Antibody 1 x 600µl 1 x 600µl 10X Human Frataxin Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 1 unit 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 6 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml 1 x 50ml Antibody Diluent 4BI 1 x 6ml 1 x 6ml Human Frataxin Lyophilized Recombinant Protein 2 vials 2 vials Plate Seal 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 12ml 0 x 0ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 0 x 0ml Stop Solution 1 x 12ml 2 x 12ml TMB Development Solution 1 x 12ml 2 x 12ml -
研究分野
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関連性
Function: Promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe2+ to proteins involved in these pathways. May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe2+ to Fe3+; the oligomeric form but not the monomeric form has in vitro ferroxidase activity. May be able to store large amounts of iron in the form of a ferrihydrite mineral by oligomerization; however, the physiological relevance is unsure as reports are conflicting and the function has only been shown using heterologous overexpression systems. Modulates the RNA-binding activity of ACO1. -
細胞内局在
Cytoplasmic and Mitochondrial -
別名
- CyaY
- FA
- FARR
see all -
参照データベース
- Entrez Gene: 2395 Human
- Omim: 606829 Human
- SwissProt: Q16595 Human
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab176112の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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特記事項 |
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Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Interpolated concentration of native Frataxin was measured in duplicate at different sample concentrations in 96-well and 384-well plates. Undiluted samples are 50 µg/mL HeLa cell lysate. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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Example of Human Frataxin standard curve in 96-well and 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Raw data values are shown in the table
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Background-subtracted data values (mean +/- SD) are graphed.
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Background subtracted data from duplicate measurements are plotted.
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Interpolated values of Frataxin are plotted for the indicated cell lines based on a extract load of 10 µg/mL.
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Transformed B lymphocyte cells from Friedreich's Ataxia (FRDA) samples, due to a stable homozygous GAA repeat insertion, are compared to heterozygous carrier B lymphocyte cells and control B lymphocyte cells. B lymphocyte cell extracts were analyzed across a 7-point titration (0.1-100 µg/mL) and frataxin levels were interpolated from the standard curve. Average interpolated values of Frataxin are plotted.
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Standard curve generated using Abcam's Frataxin Human SimpleStep ELISA™ Kit (ab176112) with human brain tissue homogenate mixed in different proportions with mouse brain tissue homogenate. ab176112 did not cross-react with mouse frataxin. Both kinetic and endpoint readouts were used sequentially for a larger dynamic range. ab110353, 12ng human frataxin full length protein was also used to extended the standard curve to 12ng/ml.
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Recombinant Frataxin was measured in biological samples in a 2-fold dilution series
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab176112 は 5 報の論文で使用されています。
- Belbellaa B et al. High Levels of Frataxin Overexpression Lead to Mitochondrial and Cardiac Toxicity in Mouse Models. Mol Ther Methods Clin Dev 19:120-138 (2020). PubMed: 33209958
- Britti E et al. Frataxin-deficient neurons and mice models of Friedreich ataxia are improved by TAT-MTScs-FXN treatment. J Cell Mol Med 22:834-848 (2018). PubMed: 28980774
- Khdour OM et al. Lipophilic methylene blue analogues enhance mitochondrial function and increase frataxin levels in a cellular model of Friedreich's ataxia. Bioorg Med Chem 26:3359-3369 (2018). PubMed: 29773347
- Piguet F et al. Rapid and Complete Reversal of Sensory Ataxia by Gene Therapy in a Novel Model of Friedreich Ataxia. Mol Ther 26:1940-1952 (2018). PubMed: 29853274
- Kemp KC et al. Cytokine therapy-mediated neuroprotection in a Friedreich's ataxia mouse model. Ann Neurol N/A:N/A (2016). Sandwich ELISA ; Human . PubMed: 28009062