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AB327301

Human FLT-3 Ligand ELISA Kit, Chemiluminescent

Human FLT-3 Ligand ELISA Kit, Chemiluminescent

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Human FLT-3 Ligand ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human FLT-3 Ligand with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.37 - 800 pg/ml with a sensitivity of 0.13 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

別名を表示する

Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine, FLT3LG

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Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)
  • sELISA

Supplier Data

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Flt-3 Ligand concentration was determined to be 63.87 pg/mL with a range of 26.63 – 105.1 pg/mL.

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)
  • sELISA

Supplier Data

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)

Interpolated concentrations of native Flt-3 Ligand in human PBMC cell culture supernatant samples. Human PBMC were cultured in the presence (stimulated) or absence (unstimulated) of 1.5% PHA-M.

Human peripheral blood mononuclear cells (PBMC) were cultured in the presence (stimulated) or absence (unstimulated) of 1.5% PHA-M for 36 hours. The concentrations of Flt-3 Ligand were measured in duplicates interpolated from the Flt-3 Ligand standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated PBMC supernatant 100% and unstimulated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Flt-3 Ligand concentration was determined to be 22.72 pg/mL in neat PHA-M stimulated PBMC cell culture supernatant 3.742 pg/mL in neat unstimulated PBMC cell culture supernatant and undetectable in naive cell culture media (data not shown).

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)
  • sELISA

Supplier Data

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)

Interpolated concentrations of native Flt-3 Ligand in human serum and plasma samples.

The concentrations of Flt-3 Ligand were measured in duplicates interpolated from the Flt-3 Ligand standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50% plasma (citrate) 50% plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Flt-3 Ligand concentration was determined to be 65.35 pg/mL in neat serum 57.01 pg/mL in neat plasma (citrate) 54.11 pg/mL in neat plasma (heparin) and 64.57 pg/mL in neat plasma (EDTA).

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)
  • sELISA

Lab

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)

Standard curve comparison between the Chemiluminescent Human Flt-3 Ligand SimpleStep ELISA and colorimetric Human Flt-3 Ligand SimpleStep ELISA (ab254507) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)
  • sELISA

Lab

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)

Dynamic range comparison between the Chemiluminescent Human Flt-3 Ligand SimpleStep ELISA, colorimetric Human Flt-3 Ligand SimpleStep ELISA (ab254507), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

検出方法

Luminescent

サンプルタイプ

Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma

交差種

Human, Monkey

アッセイタイプ

Sandwich

Quantitative
検出感度

= 0.13 pg/mL

検出範囲

0.37 - 800 pg/mL

アッセイ時間

55m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Human FLT-3 Ligand ELISA Kit, Chemiluminescent (ab327301) is a rapid single-wash sandwich 55-min ELISA to measure Human FLT-3 Ligand in Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma with a sensitivity of 0.13 pg/mL.

How the assay works

Human FLT-3 Ligand ELISA Kit, Chemiluminescent (ab327301) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human FLT-3 Ligand (ab327301) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Flt-3 Ligand antibody [EPR22288-52] - BSA and Aside free (Capture) ab259725
- Anti-Flt-3 Ligand antibody [EPR22288-201] - BSA and Aside free (Detector) ab259726
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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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回収率

[ { "sample": "Cell culture supernatant", "range": "94 - 97 %", "average": "= 95" }, { "sample": "Serum", "range": "93 - 103 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "87 - 97 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "93 - 101 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "93 - 101 %", "average": "= 97" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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製品プロトコール

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ターゲットの情報

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. Required for the development of B cells, and dendritic cells (DCs).
See full target information FLT3LG
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websiteProtocolBooklet
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