AB108848

Human Fibronectin ELISA Kit

Name: Human Fibronectin ELISA Kit (ab108848) | Abcam
Brand: Abcam Limited
SKU: AB108848
Price: 142000 JPY
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(4 Publications)

Human Fibronectin ELISA Kit is a Competitive ELISA for the measurement of Human Fibronectin in Human in Biofluids samples.

別名を表示する

FN, FN1, Fibronectin, Cold-insoluble globulin, CIG

2 Images

Competitive ELISA - Human Fibronectin ELISA Kit (AB108848)

cELISA

Lab

Competitive ELISA - Human Fibronectin ELISA Kit (AB108848)

Fibronectin measured in various samples showing quantity (microgram) per mL of tested sample

cELISA

Unknown

Competitive ELISA - Human Fibronectin ELISA Kit (AB108848)

Representative Standard Curve using ab108848.

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma, Serum

交差種

Human

アッセイタイプ

Competitive

検出感度

= 0.61 µg/mL

検出範囲

0.781 - 50 µg/mL

アッセイ時間

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Abcam's Fibronectin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibronectin levels in plasma and serum.

A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Fibronectin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibronectin captured in plate.

Get results in 90 minutes with Human Fibronectin ELISA Kit (ab181419) from our SimpleStep ELISA^® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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精度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108848-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108848-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"1X Biotinylated Human Fibronectin (Lyophilized)", "number":"AB108848-CMP03", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108848-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Fibronectin Microplate (12 x 8 well strips)", "number":"AB108848-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108848-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Fibronectin Standard", "number":"AB108848-CMP05", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108848-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108848-CMP08", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108848-CMP07", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

Multi

保管に関する情報

Please refer to protocols

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製品プロトコール

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ターゲットの情報

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed : 34089617).. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5 : ITGB1 integrin receptor signaling.

See full target information FN1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Endocrine 83:227-241 PubMed37653215

2023

Effects and plasma proteomic analysis of GLP-1RA versus CPA/EE, in combination with metformin, on overweight PCOS women: a randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyu Liao,Xing Li,Hao Zhang,Ling Zhou,Liu Shi,Weixin Li,Rufei Shen,Guiliang Peng,Huan Zhao,Jiaqing Shao,Xiujie Wang,Zheng Sun,Hongting Zheng,Min Long

Placenta 139:112-119 PubMed37356366

2023

Early pregnancy biomarker discovery study for spontaneous preterm birth.

Applications

Unspecified application

Species

Unspecified reactive species

Rik H J Beernink,Joost H N Schuitemaker,Eva F Zwertbroek,Sicco A Scherjon,Thomas I F H Cremers

Anticancer research 36:665-71 PubMed26851022

2016

L-Carnosine Prevents the Pro-cancerogenic Activity of Senescent Peritoneal Mesothelium Towards Ovarian Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Justyna Mikuła-Pietrasik,Krzysztof Książek

Journal of photochemistry and photobiology. B, Bio 144:94-103 PubMed25732262

2015

A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Di Cerbo,Carmen Laurino,Beniamino Palmieri,Tommaso Iannitti

View all publications

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websiteProtocolBooklet

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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human Fibronectin ELISA Kit